小球藻自养、异养和混养特性的研究

目的]为小球藻的高密度培养及开发高附加值代谢产物提供基础参数。方法]从细胞生长、营养物质吸收、细胞生理生化特性等方面,研究小球藻自养、异养和混养特性。结果]在异养和混养条件下小球藻生物量显著提高,最大生物量分别是自养时的3.2、4.0倍,对葡萄糖的吸收与生长状态相对应;在不同营养方式下细胞组分存在差别,在异养和混养条件下蛋白质和碳水化合物含量降低,但总脂含量增加;与自养相比,异养和混养时叶绿素含量降低,激发能在2个光合系统间的分配比PSII/PSI降低。结论]混合培养在高密度高油脂含率小球藻培养方面具重要的应用前景。

Characteristics of Auto-, Hetero-and Mixotrophic Growth of Chlorella vulgaris

Objective] The research aimed to provide the basic parameters for the high-density culture of Chlorella vulgaris and the development of metabolic products with high added value.Method] Growth and physiological characteristics of Chlorella vulgaris of different nutrition modes were examined.Result] The biomass of Chlorella vulgaris under heterotrophic and mixotrophic cultivation was respectively 3.2 and 4.0 times under autotrophic growth.The absorption of glucose was corresponding to the growth state.Compared with autotrophic growth,under heterotrophic and mixtrophic cultivation,the content of protein and soluble carbohydrate decreased,whereas the content of total lipid increased.The chlorophyll content and the exciting energy distribution value of PSⅡ/PSⅠ of Chlorella vulgaris in heterotrophic and mixotrophic cultivation were decreased.Conclusion] Mixotrophy had potential application prospect in Chlorella vulgaris cultivation with high density and high lipid content.