| 基于HPLC法的丹参中丹参酮ⅡA的超声提取工艺优化研究 |
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| 引用本文： | 刘常金,师慧敏,张治州,张琼引.基于HPLC法的丹参中丹参酮ⅡA的超声提取工艺优化研究[J].安徽农业科学,2010,38(8):4058-4059. |
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| 作者姓名： | 刘常金 师慧敏 张治州 张琼引 |
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| 作者单位： | 1. 天津科技大学食品工程与生物技术学院,天津,300457
2. 哈尔滨工业大学(威海)海洋学院分子营养与食品安全研究室,山东威海,264209 |
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| 摘 要： | 目的]确定超声波法提取丹参（Salvia miltiorrhiza Bunge）中丹参酮IIA的最佳提取工艺条件。方法]以丹参药材为原料,在单因子试验基础上,采用正交试验对丹参酮IIA的超声波辅助提取工艺进行了优化,并用HPLC法测定了提取物中丹参酮IIA的含量。结果]优化的提取条件为：在室温下,提取剂乙醇体积分数85%,提取时间30min,料液比（M：V）1：10,提取次数4次。在优化提取条件下,提取液中丹参酮IIA的含量可达到0.388%。结论]用超声波法提取丹参酮IIA快速、高效、方便、节能,有利于规模化生产。
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| 关 键 词： | 丹参 丹参酮ⅡA 超声提取法 高效液相色谱 |
Study on Optimization of Extraction Technology of Tanshinone IIA from Salvia miltiorrhiza Buhge with Ultrasonic Extraction Method Basea on HPLC |
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| LIU Chang-jin et al.Study on Optimization of Extraction Technology of Tanshinone IIA from Salvia miltiorrhiza Buhge with Ultrasonic Extraction Method Basea on HPLC[J].Journal of Anhui Agricultural Sciences,2010,38(8):4058-4059. |
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| Authors: | LIU Chang-jin |
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| Institution: | School of Food Engineering and Biotechnology;Tianjin University of science and Technology;Tianjin 300457 |
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| Abstract: | Objective]The aim was to optimize the extraction condition of tanshinone IIA from Salvia miltiorrhiza Buhge with ultrasonic extraction method.Method] On the basis of single factor experiment,an optimum extracting technology of tanshinone IIA from S.miltiorrhiza was investigated through the orthogonal experiment under the ultrasonic wave assistance conditions,then the content of tanshinone IIA in extract was analyzed with HPLC method.Result]The optimum extraction conditions were as follows:at room tempera... |
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| Keywords: | Salvia miltiorrhiza Buhge Tanshinone IIA Ultrasonic extraction HPLC |
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