HPLC法测定指甲油中苏丹红Ⅱ·苏丹红Ⅳ的含量

目的]建立高效液相色谱法测定指甲油中苏丹红Ⅱ、苏丹红Ⅳ含量的方法。方法]采用HPLC法对指甲油中苏丹红Ⅱ、苏丹红Ⅳ进行含量测定。色谱条件为:C18柱(3.5μm,4.6 mm×150 mm),柱温30℃,流动相为乙腈-缓冲溶液梯度洗脱,流速1.0 ml/min,检测波长484 nm及514 nm切换。结果]苏丹红Ⅱ、苏丹红Ⅳ色谱分离良好,浓度与峰面积呈良好线性关系,线性范围分别为苏丹红Ⅱ0～50.26μg/ml,r=0.999 8(n=6);苏丹红Ⅳ0～49.78μg/ml,r=0.999 9(n=6)。平均加样回收率分别为95.9%(n=9,RSD=2.87%)、87.7%(n=9,RSD=0.4%)。结论]该方法测定了5批指甲油中苏丹红Ⅱ、苏丹红Ⅳ的含量,分离度好、快速、简便、重现性好。

Determination of Sudan, Sudan Ⅳ in Nail Polish by RP-HPLC

Objective ] To establish an HPLC method for determination of Sudan II and SudanIV in nail polish. Method ] HPLC analysis was performed on C18 column （4.6 mm × 150 mm, 3.5 μm） with the acetonitrile-buffer solution as mobile phase in gradientmode, the column temperature was setup 30 ℃, the flow rate was 1.0 ml/min and the detecting wavelength was at 484 nm and 514 nm switching. Result] The linear response ranges from 0 to 50.26 μg/ml of Sudan 1I （ r = 0. 999 8, n = 6） and from 0 to 49.78 μg/ml of SudanIV （ r = 0. 999 9, n = 6） ; the recoveries of quercetin, apigenin and luteolin were 95.9% （ n = 9, RSD = 2. 87% ）, 87.7% （ n = 9, RSD = 0.40% ）, respectively. Con- clusion] All 5 samples were determined by HPLC. The method is a practicable method in determination of Sudan 11 and SudanlV in nail pol- ish.