Plackett-Burman和Box-Benhnken Design设计优化促进红松多酚合成诱导条件的研究

目的]优化促进红松多酚合成的诱导条件.方法]以红松不定芽为试验材料,通过单因素试验、Plackett-Burman设计试验和Box-Benhnken Deign设计试验研究8种诱导子（苯丙氨酸、肉桂酸、茉莉酸甲酯、水杨酸、壳聚糖、酵母提取物、镧、铕）对红松不定芽中多酚合成的影响,并且对促进多酚合成的诱导子组合条件进行优化.结果]除了肉桂酸外,其他7种诱导子都能不同程度地提高多酚含量.Plackett-Burman试验确定了3种影响多酚合成的关键因素：壳聚糖、镧和茉莉酸甲酯.Box-Benhnken Design试验对3种关键因素（壳聚糖、镧、茉莉酸甲酯）协同作用的诱导条件进行优化,优化得到的最佳诱导条件为：壳聚糖浓度79.58 mg/L、镧浓度42.21 μmol/L、茉莉酸甲酯浓度16.63 μmol/L,预测多酚含量最高可达到14.28 mg/g,实际多酚含量为13.42 mg/g,与对照组相比提高了122.19％.结论] Plackett-Burman设计结合Box-Benhnken Design响应面分析法可以很好地对促进多酚合成的诱导条件进行优化,3种诱导子（壳聚糖＋镧＋茉莉酸甲酯）协同组合比单一诱导子更有效地提高红松不定芽中多酚含量,更有利于地促进多酚的合成.

Optimization of Induction Conditions of Promoting the Synthesis of Polyphenols from Pinus koraiensis by Plackett-Burman and Box-Benhnken Design

Objective] The research aimed to optimize the induction condition of promoting the synthesis of polyphenols of Pinus koraiensis.Method] The adventitious buds of Pinus koraiensis were used to study the effects of 8 inductors on the synthesis of polyphenols by single-factor test,Plackett-Burman design test and Box-Benhnken design test.8 kinds of inductors were phenylalanine,cinnamic acid,methyl jasmonate,salicylic acid,chitosan,yeast extract,lanthanum and europium.The condition of promoting the synthesis of polyphenols inducers combination was optimized.Result] Besides cinnamic acid,7 kinds of inductors could improve the content of polyphenols in different degrees.3 kinds of key factors which influenced the synthesis of polyphenols were established by Plackett-Burman design test：chitosan,lanthanum and methyl jasmonate.The induciton conditions with 3 kinds of key factors were optimized by Box-Benhnken design test.The optimal induction condition was as follows：chitosan concentration 79.58 mg/L,lanthanum concentration 42.21 μ mol/L and methyl jasmonate 16.63 μmol/L.The predicted value of the content of polyphenols was 14.28 mg/g and its experimental value was 13.42 mg/g.Compared with the control group,the content of polyphenols was increased by 122.19％.Conclusion] The combination of Plackett-Burman design and Box-Benhnken design response surface analysis could optimize the induction condition of promoting the synthesis of polyphenols well.The combination of 3 inductors （chitosan ＋ lanthanum ＋ methyl jasmonate） could more effectively improve the content of polyphenols,be more conducive to promote the synthesis of polyphenols than a single inductor in adventitious buds of Pinus koraiensis.