| 罗汉果茎尖超低温保存后再生植株的遗传稳定性研究 |
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| 引用本文： | 刘华英,覃灵华,罗春玉,石贵玉.罗汉果茎尖超低温保存后再生植株的遗传稳定性研究[J].安徽农业科学,2010,38(2):629-631,673. |
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| 作者姓名： | 刘华英 覃灵华 罗春玉 石贵玉 |
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| 作者单位： | 广西师范大学生命科学学院,广西桂林,541004;广西师范大学生命科学学院,广西桂林,541004;广西师范大学生命科学学院,广西桂林,541004;广西师范大学生命科学学院,广西桂林,541004 |
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| 基金项目： | 广西自然科学基金项目(桂科自0542032);;广西师范大学博士启动基金项目;;广西研究生教育创新计划项目(20081-06020710M255) |
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| 摘 要： | 目的]检测罗汉果茎尖玻璃化法超低温保存后再生植株的遗传稳定性。方法]对罗汉果玻璃化法超低温保存前后的植株进行过氧化物酶（POD）和酯酶（EST）同工酶酶谱分析、染色体计数和随机扩增多态性DNA（RAPD）分子标记。结果]超低温保存前后，POD同工酶图谱上均显示9条酶带，且迁移率一致；EST同工酶图谱上均显示8条酶带，迁移率也一致；POD和EST同工酶谱、染色体数目和RAPD谱带均未发现差异。结论]玻璃化法超低温保存罗汉果种质资源安全、稳定。
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| 关 键 词： | 罗汉果 超低温保存 同工酶 染色体 RAPD |
Studies on the Genetic Stability of Regenerated Plants from Siraitia grosvenorii Shoot-tips after Cryopreservation |
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| LIU Hua-ying et al.Studies on the Genetic Stability of Regenerated Plants from Siraitia grosvenorii Shoot-tips after Cryopreservation[J].Journal of Anhui Agricultural Sciences,2010,38(2):629-631,673. |
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| Authors: | LIU Hua-ying |
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| Institution: | College of Life Science;Guangxi Normal University;Guilin;Guangxi 541004 |
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| Abstract: | Objective] The research aimed to examine the genetic stability of regenerated plants from Siraitia grosvenorii shoot-tips after cryopreservation by vitrification method.Method] Plants before cryopreservation and those regenerated from Siraitia grosvenorii shoot-tips after cryopreservation by vitrification were examined by peroxidase and esterase isoenzyme analysis.The number of chromosomes was counted and the molecule markers of random amplified polymorphic DNA(RAPD) was made.Result] Before and after cry... |
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| Keywords: | Siraitia grosvenorii Cryopreservation Isozyme Chromosome RAPD |
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