新疆红花主要栽培品种遗传多样性的RAPD分析

目的]利用RAPD技术分析新疆红花栽培品种遗传多样性。方法]提取29份新疆红花栽培品种的基因组DNA并利用所筛选的20条RAPD引物进行PCR扩增。结果]共扩增出156条带,其中144条带具有多态性,多态性条带占92.31%,说明新疆的红花具有较丰富的遗传多样性;根据引物扩增出的DNA指纹图谱,运用UPGMA分析法可将29份红花种质聚类划分为4个主要类群,该类群划分结果与红花的生态地域性可能不存相关性。结论]该方法可用于在分子水平上分析红花品种的遗传多样性。

Genetic Diversity in Main Cultivars of Safflower in Xinjiang Uighur Autonomous Region Based on RAPD Analysis

Objective] Study on the genetic diversity in main cultivars of safflower distributing in Xinjiang Uighur Autonomous Region by means of RAPD makers.Method] Genomic DNAs from 29 safflower accessions from Xinjiang Uighur Autonomous Region were extracted for PCR amplification using 20 RAPD primers.Result] Totally 156 bands were amplified,of which 144 were polymorphic(accounting for 92.31%),indicating that safflower is endowed with plentiful genetic diversity.Based on the DNA fingerprint,the 29 safflower accessions were grouped into four populations,the classification results may be not related with ecological regionality.Conclusion] RAPD technique is an available tool to analyze the genetic diversity of safflower germplasm at molecular level.