| HPLC法测定不同产地甘草中甘草苷的含量 |
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| 引用本文： | 栾海云,许勇,杨美子,张树平.HPLC法测定不同产地甘草中甘草苷的含量[J].安徽农业科学,2011,39(20):12121-12122. |
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| 作者姓名： | 栾海云 许勇 杨美子 张树平 |
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| 作者单位： | 滨州医学院药学院药物研究所,山东,烟台,264003;滨州医学院药学院药物研究所,山东,烟台,264003;滨州医学院药学院药物研究所,山东,烟台,264003;滨州医学院药学院药物研究所,山东,烟台,264003 |
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| 摘 要： | 目的]建立甘草中甘草苷含量测定的HPLC法,测定不同产地甘草中甘草苷的含量,以控制其质量。方法]HPLC色谱条件为：分析柱为Kromasil C18柱（4.6 mm×25 cm,5μm）;流动相为乙腈-0.5%醋酸溶液（V/,V 1∶4）;流速为1.0 ml/min;柱温为25℃;紫外检测波长为270 nm;采用外标法定量测定。结果]甘草苷在0.1~1.2μg范围内呈良好的线形关系（r=0.999 9）,其平均加样回收率和RSD值分别为97.86%和2.04%（n=6）。结论]该方法操作简单、准确、快速、重现性好,可作为甘草苷的定量分析方法。
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| 关 键 词： | 甘草(RADIX RHIZOMA GLYCYRRHIZAE) 甘草苷 高效液相色谱法 质量控制 |
Deterntination of Contents of Liquiritin in RADIX RHIZOMA GLYCYRRHIZAE from Different Producing Areas by HPLC |
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| Institution: | LUAN Hai-yun et al (Institute of Material Medical,College of Pharmacy,Binzhou Medical University,Yantai,Shandong 264003) |
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| Abstract: | Objective] To establish a method for determining contents of liquiritin in RADIX RHIZOMA GLYCYRRHIZAE from different producing areas by HPLC,so as to set up a sensitive and specific method for controlling its quality.Method] The HPLC chromatographic conditions were as follows: column of Kromasil C18(4.6 mm×25 cm,5 μm),mobile phase of acetonitrile-0.5% acetic acid(V/V,1∶4);flow rate of 1.0 ml/min,column temperature of 25 ℃;detection wavelength of 270 nm;external standard method was adopted in quantitative determination.Results] The liquiritin presented a good linear relation with peak areas in a range of 0.1 to 1.2 μg(r=0.999 9),whose average recovery and RSD were 97.86% and 2.04%(n=6),respectively.Conclusion] The method established in this study was simple,convenient and rapid with good repeatability which can be used in quantitative analysis of RADIX RHIZOMA GLYCYRRHIZAE. |
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| Keywords: | RADIX RHIZOMA GLYCYRRHIZAE Liquiritin HPLC Quality control |
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