猕猴桃组培苗生根培养的研究

目的]为建立和完善猕猴桃快速繁殖体系提供理论依据。方法]以美味猕猴桃无根组培苗为试材,研究培养基、IBA浓度和活性炭对其生根的影响。结果]1/2MS培养基是最适合诱导猕猴桃组培苗生根的基本培养基,生根率、平均生根数分别是MS培养基的5.05、6.46倍。当IBA浓度为1.0mg/L时,猕猴桃组培苗的生根率为84.4%,生根数为3.82条/株,根平均长度为1.96cm,且植株生长健壮。活性炭对猕猴桃组培苗生根有明显的促进作用,当培养基中加入0.3%的活性炭时,猕猴桃组培苗的生根率达95.6%,生根数达4.53条/株,根平均长度达2.67cm。结论]猕猴桃组培苗最适宜的生根培养基为1/2MS培养基+IBA1.0mg/L+0.3%活性炭。

Study on Rooting Culture in Tissue Culture Seedlings of Actindia deliciosa

Objective] The study aimed to provide the theoretical base for setting up and perfect the rapid propagation system of Actindia deliciosa.Method] With the rootless tissue culture seedlings of A. deliciosa as the explants, the effects of medium, IBA concn. and activated carbon on its rooting were studied. Result] 1/2 MS was the optimum basic medium to induct the rooting of A. deliciosa tissue culture seedlings, on which, the rooting rate, mean rooting number were 5.05, 6.46 times of that on MS medium. When IBA was 1.0 mg/L, the rooting rate, mean rooting number of the tissue culture seedlings were 84.4%, 3.82/plant,and 1.96 cm, resp. and the plantlet grown strongly. The activated carbon could obviously promote the rooting of the tissue culture seedlings. When 0.3% activated carbon was added into the medium, the rooting rate, mean rooting number of the tissue culture seedlings were 95.6%, 4.53/plant and 2.67 cm, resp. Conclusion] The optimum medium for rooting of A. deliciosa tissue culture seedlings was 1/2 MS medium IBA 1.0 mg/L 0.3% activated carbon.