不同激素对金花茶圈枝繁殖生根的影响

分析NAA、IBA及2种激素等体积混合溶液对金花茶圈枝繁殖生根成活率的影响。结果表明:采用萘乙酸和吲哚丁酸的等体积混合激素溶液1 000~1 500 mg/L能明显提高金花茶圈枝繁殖苗的生根率,达到95%以上。该技术简化了操作程序,可节省人力,在基质方面也节约了生产成本,可直接应用于金花茶苗木大规模生产。     

Factors affecting in vitro adventitious shoot formation on internode explants of Citrus aurantium L. cv. Brazilian

The in vitro formation of newly formed adventitious buds and shoots from internodal branch segments was studied on 12-month-old plants of Citrus aurantium L. cv. Brazilian. The effects of 6-Benzyladenine (BA) and α-Naphthalene acetic acid (NAA) treatments were evaluated on adventitious bud and shoot regeneration. High rates of bud initiation and shoot development were obtained both with BA supplemented medium, in the range from 1 mg L⁻¹ to 3 mg L⁻¹, and with 0.1 mg L⁻¹ NAA supplemented medium. NAA concentrations above 1 mg L⁻¹ significantly reduced bud initiation and shoot elongation. The results obtained using different in vitro culture vessels such as Petri dishes, tubes and glass culture jars were compared. The highest adventitious bud induction was observed in Petri dishes for internodes cultured in 2 mg L⁻¹ BA supplemented medium, with 95% responsive explants forming 9.0 ± 2.4 adventitious buds. The adventitious buds observed in Petri dishes reached a maximum height of 1 mm, with no further development, while some of the adventitious shoots cultured in tubes and glass culture jars grew over 1 cm in height. A shoot regeneration gradient of the internodes collected along the branch axis was noticed, with basal ones exhibiting higher regeneration frequency.     

Variation in Shoot Anatomy and Rooting Behaviour of Stem Cuttings in Relation to Age of Donor Plants in Teak (Tectona grandis Linn. f.)

Shoot anatomical features viz., number of vessel elements, width and length of vessel and fibre elements were studied in relation to juvenility/maturity of teak (Tectona grandis Linn. f.). The findings show significant differences in these features, which indicates that variation in shoot anatomy can be used as a reliable indicator of juvenility/maturity in teak. The effect of age of donor plants and application of auxins on adventitious root formation in single noded leafy stem cuttings of teak was also investigated. Aging of donor trees suppressed rooting and sprouting of cuttings, but increased callus formation at the base of cuttings. Per cent rooting and per cent sprouting in cuttings decreased as age of donor plants increased from 2-months to 15-years and up to 30-years. IBA treatment promoted per cent rooting as well as per cent sprouting, NAA promoted only rooting and it suppressed sprouting. The effect of auxin treatment on rooting and sprouting of teak stem cuttings varied with the age of donor plants. Auxin and its concentration, which suppressed rooting and sprouting in cuttings of younger donor plants, actually promoted rooting when cuttings were taken from more mature donor plants. It appears that the auxin requirement for causing and promoting rooting in teak cuttings increased with increasing age of donor plants. The higher auxin requirement for causing and promoting rooting in cuttings of older trees may be due to a decrease in the content on endogenous auxins or decreased sensitivity of aging tissues to rooting promoters and /or accumulation of inhibitory substances which inhibit rooting.     

Rooting of baldcypress cuttings

A test of the rooting performance and growth of cuttings from 1-year-old and 25-to 50-year old baldcypress (Taxodium distichum L.) branch tips is reported. Rooting of cuttings from one-year-old trees averaged 75% (0.1% IBA treatment) and 88% (control), cuttings from 25 to 50-year-old trees averaged 12% in both the IBA treatment and the control treatment. Indole-3-butyric acid at a 0.1% concentration did not enhance rooting, but resulted in a significant increase in shoot dry weight on cuttings from one-year-old trees. The results of this experiment suggest that cuttings from mature baldcypres trees have the potential to root, thereby, additional rooting techniques such as girdling and stump sprouts is worthy of pursuing.     

两种外源激素在白掌组织培养不同阶段的剂量研究

文章研究了不同浓度的细胞分裂素6-BA对白掌外植体诱导、愈伤组织增殖、不定芽增殖及生长素IBA对诱导生根的影响。结果表明：初代培养基以1／2MS CM100ml／L 蔗糖30g／L 琼脂6g／L 6-BA 1.0mg／L效果最好；在培养基MS NAA 0．1mg／L 蔗糖30g／L 琼脂6g／L中添加6-BA 1.0mg／L愈伤组织和不定芽的增殖倍数最高；在培养基1／2MS 活性炭2g／L 蔗糖30g／L 琼脂6g／L中添加2．0mg／L IBA可显著提高生根率。     

Vegetative propagation of Colutea istria Mill. from leafy stem cuttings

This study investigates the optimal conditions for the vegetative propagation of the multipurpose leguminous shrub Colutea istria from leafy stem cuttings. The conditions examined included 1) the origin of the cutting (apical, medial or basal), 2) indole-3-butyric acid (IBA) treatment (0, 50, 200 or 400 mg/l), 3) the relative humidity of the propagation environment (high or low), 4) wounding base of cutting, and 5) the season in which the cutting was taken. The rooting ability of the cuttings was significantly influenced by all these factors. Winter cuttings obtained from the middle and base of the branch showed a greater rooting capacity than those harvested from the apical area, while autumn cuttings harvested from the middle and apical areas of the branch showed a greater rooting capacity than those harvested from the base. Winter cuttings showed the best rooting capacity than autumn cuttings. IBA treatment (200 mg/l), high humidity (98-100%), and wounding increased the rooting capacity of the cuttings. The sprout of new leaves was higher in basal cuttings, while IBA and wounding also significantly enhanced the root number of rooted cuttings. A method for vegetatively propagating C. istria plants is given.     

吲哚丁酸和萘乙酸在茶树短穗扦插上的应用效果

用不同浓度的吲哚丁酸(IBA)和萘乙酸(NAA)组合处理茶树短穗,进行扦插,结果表明:IBA和NAA及其交叉组合对扦插成活率、发根率、发根条数的差异均未达到显著;IBA对最长根长的效果显著,且IBA40×10-6明显优于其他浓度处理。     

Air layering and its potential in propagating Uapaca kirkiana: a fruit tree from the miombo woodland,Tanzania

The potential to propagate Uapaca kirkiana through air layering was investigated at Igumbilo forest, Tanzania. The objective was to determine the effect of time of setting air layers and use of indole-3-butyric acid (IBA) to promote rooting. Air layers were initiated on young shoots, 1-to 2-years old, growing on mature trees. After root initiation, they were detached, potted and reared at the nursery for a further three months. Thereafter they were harvested and assessed. Two factors were investigated: effect of time at which air layers were initiated (June, September and December), and influence of IBA at 50, 100 and 150 mg l^?1concentrations. Varied rooting successes were realised, being influenced by time at which air layers were initiated, IBA concentration or both. Optimal rooting of 82.5% was realised in June-initiated air layers treated with 50 mg l^?1 IBA. Air layers initiated in December and treated with the control had the poorest rooting (46.7%). Application of 100 mg l^?1 IBA during June significantly improved the number of roots, root length and root biomass produced. The increase in rooting ability during June is partly linked to better nutritive status, since most plants at this time have sufficient food reserves acquired during the active photosynthetic rainy period of January–May. The period is also associated with minimal plant developmental activities such as budding and flushing, which are usually antagonistic to rooting. Thus, air layering is a potential technique to propagate U. kirkiana, and can contribute effectively to capture desirable traits rapidly disappearing because of ongoing deforestation and maintain the desired fruit tree attributes once attained. Further studies are recommended to determine its cost effectiveness in relation to other vegetative techniques.     

NAA和IBA对福建茶扦插生根的影响

用300 mg/L NAA和300 mg/L IBA处理福建茶1 h、2 h、3 h、4 h,用600 mg/L NAA和600 mg/L IBA处理福建茶1 min、5 min、10 min、20 min,60 d起苗观察生根情况。结果表明,300 mg/LNAA和300 mg/L IBA处理福建茶,NAA处理3 h和IBA处理4 h效果最好,生根率83%和87%;600 mg/L NAA和600 mg/L IBA处理福建茶,NAA处理5 min和IBA处理10 min效果最好,生根率83%和90%。     

红花槭扦插生根特性和解剖学研究

以红花槭‘十月光辉’1年生带顶芽、1年生无顶芽和2年生硬枝为插穗,选用M1(河沙与珍珠岩的体积比为1∶1)、M2(河沙与蛭石的体积比为1∶1)、M3(珍珠岩与蛭石的体积比为1∶1)和M4(河沙、珍珠岩、蛭石的体积比为1∶1∶1)4种不同的扦插基质,施加不同质量浓度的吲哚丁酸(IBA)和萘乙酸(NAA)进行扦插试验,研究插穗类型、基质种类和外源激素对红花槭扦插生根的影响,并对扦插过程中不定根发育形成过程进行组织学观察。结果表明:M4基质在生根率(69.44%)、平均生根数量(14.31条)和平均根长(5.40cm)方面均表现最优,为适宜的扦插基质;1年生插穗生根率和平均根数均高于2年生插穗的,平均根长低于2年生插穗的;1年生带顶芽与1年生无顶芽插穗的扦插生根率差异不显著(P0.05);500mg/L IBA+500mg/LNAA处理效果最好,生根率达75.56%,平均生根数量为15.68条,平均根长为6.48 cm。红花槭属于诱生根原基多位点发生类型,其不定根起源于木射线与维管形成层交叉区域和韧皮部薄壁组织。