| 通过酵母双杂交筛选水稻类受体激酶CR4的胞外结合蛋白 |
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| 引用本文： | 姚清国,周二鹏,王娟,张文娜,王景翔.通过酵母双杂交筛选水稻类受体激酶CR4的胞外结合蛋白[J].安徽农业科学,2011,39(8):4427-4430. |
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| 作者姓名： | 姚清国 周二鹏 王娟 张文娜 王景翔 |
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| 作者单位： | 石家庄学院化工学院,河北石家庄,050035;石家庄学院化工学院,河北石家庄,050035;石家庄学院化工学院,河北石家庄,050035;石家庄学院化工学院,河北石家庄,050035;石家庄学院化工学院,河北石家庄,050035 |
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| 基金项目： | 2010年石家庄市科学技术研究与发展项目(10120803);2007年石家庄学院科研启动基金立项项目(2007012);2008年度石家庄学院教学改革研究项目(2008006) |
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| 摘 要： | 目的]寻找CR4的胞外结合蛋白。方法]采用OsCR4的胞外域作为诱饵蛋白,酵母双杂交筛选14 d的小苗cDNA文库。结果]得到了许多蛋白,包括一个过氧化物B（D26484）、甲硫氨酸硫氧还原酶（ABF96078）和一个未知功能的蛋白。结论]为研究CR4的信号转导机制提供了理论依据。
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| 关 键 词： | 水稻 类受体激酶 胞外结合蛋白 酵母双杂交 |
Screening of Extracellular Binding Proteins of Rice Receptor-like Kinase CR4 by the Yeast Two-hybrid |
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| Institution: | YAO Qing-guo et al (College of Chemical Engineering in Shijiazhuang University,Shijiazhuang,Heibei 050035) |
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| Abstract: | Objective] The research aimed to find the extracellular binding proteins of CR4.Method] The extracellular region of OsCR4 was as the bait protein,and the yeast two-hybrid was used to screen the seedling cDNA library which was cultivated 14 d.Result] A lot of proteins which included a peroxid B(D26484),a methionine thioredoxin reductase(ABF96078) and an unknown function protein were gained.Conclusion]It provided the theory basis for studying the signal transduction mechanism of CR4. |
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| Keywords: | Rice Receptor-like kinase Extracellular binding protein Yeast two-hybrid |
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