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分光光度法测定植酸含量
引用本文:郝红英,周,芳,周彩荣等.分光光度法测定植酸含量[J].安徽农业科学,2014(7):1907+1975-1907,1975.
作者姓名:郝红英      周彩荣等
作者单位:[1]黄河科技学院,河南郑州450063 [2]2,郑州大学,河南郑州450¨00l [3]郑州开普工程技术有限公司,河南郑州450001
摘    要:目的]为了寻找适合于标准植酸的测定及微量测定的方法。方法]采用可见分光光度法,测定植酸的含量。结果]在标准曲线下,植酸浓度在0~32μg/ml范围内与吸光度呈良好线性关系,方程为:Y=-2.284 2X+0.384 1(R=0.997 3),平均回收率为97.35%,RSD为4.82%(n=4)。结论]该方法操作简便、快速、准确,可作为植酸的定量分析方法。

关 键 词:植酸  含量  可见分光光度法

Determination on Phytic acid by Spectrophotometry
Institution:HAO Hong-ying et al ( Huanghe Science and Technology College, Zhengzhou, Henan 450063 )
Abstract: Objective] The research aimed to find the method suited to the measurement of standard phytic acid and microdetermination. Method] A method for determination of phytic acid by speetrophotometry was studied. Result] The regression equation within the range of' the standard sample concentration in 0 -32 μg/ml was Y = -2. 284 2X + 0. 384 1 with a good linearity (R = 0. 997 3 ). The average recovery rate was 97.35 % and RSD was 4.82% (n = 4). Conclusion] The method was simple, rapid, accurate and suitable for the quality evaluation of phytic acid.
Keywords:Phytic acid  Content  Spectrophotometry
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