仙茅地下茎段组织培养研究

目的]为仙茅的快速繁殖提供借鉴和依据。方法]以仙茅无菌地下茎段为外植体,在蔗糖浓度为20 g/L,琼脂用量为6.5 g/L,pH值为5.8,温度为25℃的光照条件下,设3组培养基（A愈伤组织诱导培养基MS＋6-BA 0-1.0 mg/L＋NAA 0-1.0 mg/L,B丛生芽诱导培养基MS＋6-BA 0.5-1.5 mg/L＋NAA 0.2-0.8 mg/L,C生根培养基MS＋IAA 0-0.5 mg/L）进行组培试验。结果]最适合仙茅的愈伤组织诱导培养基为MS＋6-BA 0.2-1.0 mg/L＋NAA 0.1-0.6 mg/L;丛生芽分化培养基为MS＋6-BA 0.8-1.5 mg/L＋NAA0.2-0.5 mg/L;生根培养基为MS＋IAA 0.1-0.3 mg/L。仙茅的愈伤组织诱导率为88%-93%,丛生芽诱导率为93%-96%,生根诱导率为92%-96%。结论]该研究为仙茅地下茎段的组织培养提供了试验参考和理论依据。

Study on the Tissue Culture of Curculigo orchioides Rhizome Section

Objective] The purpose of the study was to supply reference and foundation for the rapid propagation of Curculigo orchioides.Method] With aseptic C.orchioides rhizome sections as explants,under the condition with sucrose concn.of 20 g/L,algar dosage of 6.5 g/L,pH value of 5.8,temperature of 25 ℃ and illumination,3 groups of media(A: callus induction medium MS+6-BA 0~1.0 mg/L+NAA 0~1.0 mg/L,B: multiple bud clmps induction medium MS+6-BA 0.5~1.5 mg/L +NAA 0.2~0.8 mg/L and C: rooting medium MS+IAA 0~0.5 mg/L) were arranged for tissue culture experiment.Result] The most suitable callus induction medium for C.orchioides was MS+6-BA 0.2~1.0 mg/L +NAA 0.1~0.6 mg/L,the most suitable multiple bud clmps differentiation medium was MS+6-BA 0.8~1.5 mg/L +NAA 0.2~0.5 mg/L and the most suitable rooting medium was MS+IAA 0.1~0.3 mg/L.The callus induction rate of C.orchioides was 88%~93%,its multiple bud clmps induction rate was 93%~96% and its rooting induction rate was 92%~96%.Conclusion] The study provided experimental reference and theoretical basis for the tissue culture of C.orchioides rhizome sections.