尾巨桉 DH32－26组培快繁技术

目的]探索尾巨桉DH_(32-26)组培快繁技术,为其规模化生产提供参考。方法]采用组织培养手段,以尾巨桉DH_(32-26)半木质化萌发茎段为材料,对外植体诱导及无菌组织培养体系进行研究。结果]最佳外植体诱导培养基为改良MS+6-BA 1.0 mg/L+NAA 0.5mg/L,发芽率为92.3%;最佳增殖培养基为改良MS+6-BA 0.4 mg/L+NAA 0.2 mg/L,增殖个数为3.7;最佳生根培养基为改良1/2MS+IBA 0.2 mg/L+ABT 0.3 mg/L,生根率可达98.3%。结论]该研究获得了尾巨桉初代诱导、增殖和生根适宜培养基和培养条件,初步达到工厂化生产要求。

Rapid Propagation Technology of Eucalyptus grandis ×E.urophylla DH32-26

Objective] To research the rapid propagation technology of Eucalyptus grandis ×E.urophylla DH32 -26 ,and to provide references for the large -scale production.Method] Half lignification ger mination stem section of Eucalyptus grandis ×E.urophylla DH32 -26 was used as the research material.By using the means of tissue culture,we carried out explant induction and sterile tissue culture system in order.Result] The optimal explant induction medium was improved MS +6 -BA 1.0 mg /L +NAA 0.5 mg /L,with the ger mination rate being more than 92.3%. The optimal proliferation medium was improved MS +6 -BA 0.4 mg /L +NAA 0.2 mg /L,with number of bud proliferates was 3.7.The best me-dium that the root induce was improved 1 /2 MS +IBA 0.2 mg /L +ABT 0.3 mg /L,the rooting rate was 98.3%.Conclusion] This research obtains the optimal culture mediums and cultivation conditions of initial induction,proliferation and rooting of Eucalyptus grandis ×E.urophylla DH32 -26 ,so as to preli minarily meet the requirements of industrialized production.