铁线莲组培快繁技术研究

目的]研究不同激素浓度对铁线莲诱导、增殖和生根的影响,建立铁线莲组培快繁技术。方法]以铁线莲茎段为外植体,在MS培养基中加入不同浓度的6-BA、NAA和IBA,研究不同激素浓度对其腋芽诱导分化、诱导芽增殖和继代苗生根的影响。结果]铁线莲腋芽诱导效果以MS+6-BA 1.0 mg/L+NAA 0.2 mg/L为最佳;增殖培养以MS+6-BA 1.5 mg/L+NAA 0.50 mg/L为最佳;继代苗生根培养以MS+6-BA 1.5 mg/L+IBA 2.0 mg/L最佳。结论]建立了铁线莲组培快繁技术体系,为其规模化生产提供理论依据。

Study on the Tissue Culture Rapid Propagation Technique of Clematis florida

Objective] The aim was to study effects of different hormone concentration on induction, proliferation and rooting of Clematis flori-da, to establish tissue culture rapid propagation technique of Clematis florida.Method] With Clematis florida stem segments as explants, adding different concentrations of 6-BA, NAA, IBA growth hormone in MS culture medium , effects of different hormone concentration on induction dif-ferentiation, bud proliferation, and rooting culture of successive seedling were studied.Result] Clematis florida axillary bud induction effects in MS +6-BA 1.0mg 0.2 mg/L way was the best;proliferation cultured in MS +6-BA 1.5 mg/L+NAA 0.5 mg /L treatment was the best;suc-cessive generation of rooting culture in process S3: MS+6-BA 1.5 mg/L+IBA 2.0 mg/L was the best.Conclusion] The tissue culture rapid propagation technique system of Clematis florida is established, which can provide theoretical basis for large-scale production.