| 贺州大肉姜的组培快繁技术研究 |
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| 引用本文： | 邓年方,潘百明,韦学丰.贺州大肉姜的组培快繁技术研究[J].安徽农业科学,2010,38(11):6013-6014,6035. |
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| 作者姓名： | 邓年方 潘百明 韦学丰 |
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| 作者单位： | 贺州学院化学与生物工程系,广西贺州,542800;贺州学院化学与生物工程系,广西贺州,542800;贺州学院化学与生物工程系,广西贺州,542800 |
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| 基金项目： | 广西贺州市科技局科技攻关项目，桂东特色资源重点建设实验室项目 |
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| 摘 要： | 目的]研究大肉姜组培快繁技术,为贺州大肉姜规模化生产提供技术支持。方法]以贺州大肉姜的芽作为外植体,研究不同消毒液和激素组合对外植体污染率、芽分化和生根的影响。结果]外植体用0.1%HgCl2消毒12min效果最好,诱导芽分化的最佳培养基为MS＋2.0mg/L6-BA＋0.1mg/LNAA,最佳生根培养基为1/2MS＋1.0mg/LNAA,生根率可达100%。结论]该组培快繁技术可用于贺州大肉姜的规模化生产。
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| 关 键 词： | 贺州大肉姜 外植体 组织培养 |
Tissue Culture and Rapid Propagation of Hezhou Ginger |
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| Institution: | DENG Nian-fang et al (Department of Chemical and Biological Engineering,Hezhou University,Hezhou,Guangxi 542800) |
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| Abstract: | Objective] To study the tissue culture and rapid propagation technology of Hezhou ginger and provide technical support for large-scale production.Method] Hezhou ginger (Zingiber officinale Rosc.) buds were used as explants.The effects of different disinfectants and hormonal combinations on contamination rate,bud differentiation and rooting were studied.Result] The disinfection efficiency of explants were better with 0.1% HgCl2 for 12 min.The optimal medium for bud differentiation was MS+2.0 mg / L 6-BA+0.1 mg/L NAA.The best medium for rooting was 1/2MS+1.0 mg/L NAA,and the rooting rate was up to 100%.Conclusion] The tissue culture and rapid propagation technology can be used for large-scale production of Hezhou ginger. |
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| Keywords: | Hezhou ginger Explant Tisssue culture |
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