紫外分光光度法测定淫羊藿中总黄酮的含量 |
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引用本文: | 王东升,张世栋,苗小楼,董书伟,严作廷.紫外分光光度法测定淫羊藿中总黄酮的含量[J].安徽农业科学,2013(17):7471-7472. |
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作者姓名: | 王东升 张世栋 苗小楼 董书伟 严作廷 |
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作者单位: | 中国农业科学院兰州畜牧与兽药研究所,农业部兽用药物创制重点实验室,甘肃省中兽药工程技术研究中心,甘肃省新兽药工程重点实验室,甘肃兰州730050;中国农业科学院兰州畜牧与兽药研究所,农业部兽用药物创制重点实验室,甘肃省中兽药工程技术研究中心,甘肃省新兽药工程重点实验室,甘肃兰州730050;中国农业科学院兰州畜牧与兽药研究所,农业部兽用药物创制重点实验室,甘肃省中兽药工程技术研究中心,甘肃省新兽药工程重点实验室,甘肃兰州730050;中国农业科学院兰州畜牧与兽药研究所,农业部兽用药物创制重点实验室,甘肃省中兽药工程技术研究中心,甘肃省新兽药工程重点实验室,甘肃兰州730050;中国农业科学院兰州畜牧与兽药研究所,农业部兽用药物创制重点实验室,甘肃省中兽药工程技术研究中心,甘肃省新兽药工程重点实验室,甘肃兰州730050 |
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基金项目: | "十二五"国家科技支撑计划项目 |
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摘 要: | 目的]中药淫羊藿中总黄酮的含量测定。方法]试验采用紫外分光光度法,以淫羊藿苷为对照品,以无水甲醇作空白对照,在270 nm波长处测定吸光度,计算总黄酮含量。结果]淫羊藿苷浓度在1.68~10.08μg/ml范围内与吸光度的线性关系良好,R=0.999 4,总黄酮的平均回收率为100.36%,RSD为1.16%,淫羊藿中总黄酮的含量为(8.09±0.01)%。结论]采用紫外分光光度法测定淫羊藿总黄酮的含量,操作快速,简便,准确。
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关 键 词: | 淫羊藿(HERBA EPIMEDII) 总黄酮 含量 紫外分光光度法 |
Determination on the Content of Total Flavonoids in HERBA EPIMEDH by Ultraviolet Spectrometry |
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Institution: | WANG Dong-sheng;Key Laboratory of Veterinary Pharmaceutical Development,Ministry of Agriculture/Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS/ Engineering&Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province/Key Laboratory of New Animal Drug Project of Gansu Province; |
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Abstract: | Abstract Objective ] To determine the content of total flavonoids in HERBA EPIMEDII. Method ] Icariin was standard preparation and meth- anol was blank to measure the absorbance of the test solution and the reference solution at 270 nm wavelength with UV speetophotometry. IRe- suit] The results showed that the linear range for the content of icariin was 1.68 - 10.08 p.g/ml, R =0. 999 4, the average recovery rate of total flavonoids was 100.36% and RSD was 1.16%, the content of total flavonids of HERBA EPIMEDII was(8.09 :i:0.01 ) %. Conclusion] These indicated that method of determination of total flavonoids in HERBA EPIMEDII with UV spectrophotometry is rapid, accurate, convenient and oractical. |
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Keywords: | HERBA EPIMEDII Total flavonoids Content UV spectrophotometry |
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