| 文冠果细胞悬浮培养技术研究 |
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| 引用本文： | 柳金凤,吴建华,闵丽霞.文冠果细胞悬浮培养技术研究[J].安徽农业科学,2009,37(32):15706-15708. |
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| 作者姓名： | 柳金凤 吴建华 闵丽霞 |
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| 作者单位： | 宁夏林业研究所(有限公司),种苗生物工程国家重点实验室,宁夏银川,750004;宁夏林业研究所(有限公司),种苗生物工程国家重点实验室,宁夏银川,750004;宁夏林业研究所(有限公司),种苗生物工程国家重点实验室,宁夏银川,750004 |
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| 摘 要： | 目的]建立文冠果体细胞胚胎发生和快速成苗的悬浮培养体系，为文冠果的产业化和规模化发展提供参考。方法]以文冠果新旧种子为材料，诱导体胚发生，进行文冠果细胞悬浮培养。结果]适合胚性愈伤诱导的最佳培养基为MS＋6-BA1．0mg／L＋NAA0．5mg／L＋2，4-D0．5mg／L＋3％蔗糖；适合胚性愈伤组织不定芽诱导培养的最佳诱导培养基为MS＋6一BA1．0mg／L．4-NAA1．0mg／L＋3％蔗糖，且萌芽率最高；适合单细胞团再生植株培养的最佳培养基为MS＋6-BA0．5mg／L＋NAA0．2mg／L＋3％蔗糖。结论]NAA和2，4-D混合使用有利于胚性愈伤组织的形成；一定浓度的NAA对文冠果愈伤组织萌芽培养有较大的促进作用。
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| 关 键 词： | 文冠果 体细胞胚胎发生 悬浮培养 |
Study on Cell Suspension Culture Technology of Xanthoceras sorbifolia |
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| LIU Jin-feng et al ,State Key Laboratory of the Seedling , Bioengineering,Yinchuan,Ningxia.Study on Cell Suspension Culture Technology of Xanthoceras sorbifolia[J].Journal of Anhui Agricultural Sciences,2009,37(32):15706-15708. |
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| Authors: | LIU Jin-feng State Key Laboratory of the Seedling Bioengineering Yinchuan Ningxia |
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| Institution: | LIU Jin-feng et al (Ningxia Forestry Institute (LTD.CO),State Key Laboratory of the Seedling , Bioengineering,Yinchuan,Ningxia 750004) |
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| Abstract: | Objective] To establish suspension culture system of cell embryogenesis and fast seedling formation of Xanthoceras sorbifolia,and provide reference for the developments of industrialization and scale.Method] Taking new and old seed of Xanthoceras sorbifolia as material,inducing embryogenesis,the cell suspension culture of Xanthoceras sorbifolia was carried out.Result] The optimal medium of MS+6-BA 1.0 mg/L+NAA 0.5 mg/L+2,4-D 0.5 mg/L+3% sucrose was suitable for embryogenic callus induction.The optimal me... |
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| Keywords: | Xanthoceras sorbifolia Somatic embryogenesis Suspension culture |
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