| 利用RAPD分子标记分析东北地区杂草稻的亲缘关系 |
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| 引用本文： | 吴明根,袁晓丹,柳参奎,金东淳.利用RAPD分子标记分析东北地区杂草稻的亲缘关系[J].安徽农业科学,2007,35(1):49-51. |
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| 作者姓名： | 吴明根 袁晓丹 柳参奎 金东淳 |
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| 作者单位： | 延边大学农学院,吉林龙井,133400;东北林业大学,黑龙江哈尔滨,150040 |
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| 基金项目： | 国家自然科学基金项目(2004,30360063) |
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| 摘 要： | 采用随机扩增多态性DNA(RAPD)技术,对东北地区杂草稻生态型以及普通水稻共15个样品进行亲缘关系的分析.所分析的10个随机引物共扩增出了93条DNA片段,其中多态性条带为36条,多态性比例为38.71%,利用93个RAPD标记,在MEGA2.1软件中,计算遗传距离,建立UPGMA聚类图.结果表明:RAPD分子标记可以将杂草稻与普通稻、杂草稻不同生态型之间分开;杂草稻之间形态相似、地理分布相近的物种聚类在一起.
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| 关 键 词： | 杂草稻 RAPD 遗传距离 |
| 文章编号： | 0517-6611（2007）01-00049-03 |
| 修稿时间： | 2006-09-28 |
Analysis of Genetic Relationship of Weedy Rice by RAPD Molecular Makers in Northeast Area |
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| WU Ming-gen ct al.Analysis of Genetic Relationship of Weedy Rice by RAPD Molecular Makers in Northeast Area[J].Journal of Anhui Agricultural Sciences,2007,35(1):49-51. |
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| Authors: | WU Ming-gen ct al |
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| Institution: | Agricultural College, Yanbian University, Longjing, Jilin 133400 |
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| Abstract: | The genetic relationship of 15 varieties including weedy rice and cultivated rice in Northeast area was analyzed by RAPD.93 DNA fractions were amplified,among which 36 fractions were polymorphism,and the rate was 38.71%.The genetic distance was calculated.UPGMA was formed by 93 markers in MEGA2.1 soft ware.The results indicated that the weedy rice and cultivated rice,different biotypes of weedy rice could be separated by RAPD.The weedy rice with the similar characters and geographic distribution clustered together. |
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| Keywords: | Weedy rice RAPD Genetic distance |
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