| 丽格海棠再生体系建立的研究 |
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| 引用本文： | 张云峰,李静,邢宇俊,季勤.丽格海棠再生体系建立的研究[J].安徽农业科学,2007,35(3):663-664. |
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| 作者姓名： | 张云峰 李静 邢宇俊 季勤 |
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| 作者单位： | 淮阴师范学院生物系,江苏淮安,223300;淮阴师范学院生物系,江苏淮安,223300;南京农业大学生命科学学院,江苏南京,210095 |
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| 摘 要： | 对丽格海棠幼嫩叶片和叶柄离体培养与植株再生过程进行了研究,建立了丽格海棠的再生体系.该体系是以1 cm2左右带叶脉的幼嫩叶切片为外植体;丛生芽诱导培养基为MS+6-BA 0.5 mg/L+NAA 0.5 mg/L或MS+6-BA0.5 mg/L+NAA0.1 mg/L+4%芦荟原汁,接种培养30～40 d;丛生芽继代培养基为MS+6-BA 0.05 mg/L+NAA 0.1 mg/L,培养时间20 d;生根培养基为1/2 MS+IBA 0.5 mg/L,培养时间30 d.该体系的建立对丽格海棠种苗快速繁殖和规模化生产将有着重要的指导意义.
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| 关 键 词： | 丽格海棠 再生体系 外植体 丛生芽 芦荟原汁 |
| 文章编号： | 0517-6611（2007）03-00663-02 |
| 修稿时间： | 2006-10-22 |
Study on the Establishment of Rieger Begonia Regeneration System |
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| ZHANG Yun-feng et al.Study on the Establishment of Rieger Begonia Regeneration System[J].Journal of Anhui Agricultural Sciences,2007,35(3):663-664. |
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| Authors: | ZHANG Yun-feng |
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| Institution: | Department of Biology, Huaiyin Teachers College, Huaian, Jiangsu 223300 |
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| Abstract: | Plant regeneration system from Rieger begonia leaf culture was studied and established.The results showed that petiole Rieger begonia leaf with main vein(1 cm2) was used as explants;the optimum medium for adventitious shoot induction was MS + 6-BA 0.5 mg/L + NAA 0.5 mg/ L or MS + 6-BA 0.5 mg/L + NAA 0.1 mg/ L + 4% aloe juice,and abundant shoots were obtained after 30～40 d from pre-cultured explants on the medium.MS + 6-BA 0.05 mg/L + NAA 0.1 mg/L and 1/2MS + IBA 0.5 mg/L was suitable medium for shoot multiplication and rooting,respectively. |
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| Keywords: | Rieger begonia Regeneration system Explant Adventitious shoot Aloe juice |
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