福禄桐组培快繁成苗技术研究

目的]研究圆叶福禄桐茎段为外植体离体培养的快繁技术。方法]以圆叶福禄桐茎段为外植体离体培养,探讨最佳培养基配比。结果]用浓度0.1%升汞溶液对福禄桐材料进行消毒,时间8～10min效果较佳;以茎段为外植体诱导出腋芽,MS＋6-BA3.0mg/L＋NAA0.5mg/L的激素配比对诱导发芽有利,诱导率高达85.6%;增殖培养基MS＋6-BA1.5mg/L＋NAA0.1mg/L最佳;不定芽在1/2MS＋IBA1.0mg/L的生根培养基上,生根率高达94.9%,根多条、粗壮,植株长势良好、有活力;生根苗移栽在泥炭与椰糠2∶1的基质中,成活率最高。结论]该研究为圆叶福禄桐离体快繁提供了依据。

Study on Tissue Culture Seedlings of Polysics balfouriana

Objective] The research aimed to study the tissue culture seedlings of Polysics balfouriana.Method] Soft stems were used as explants and lots of medium was used to study the tissue culture regeneration potential of Polysics balfouriana.Result] The soft stems of Polysics balfouriana sterilized with 0.1% hydrargyrum for 8 to 10 minutes were suitable for explants.The best medium for inducing callus was MS+BA 3.0 mg/L+NAA 0.5 mg/L,and the inducing ratio reached 85.6%.The best medium for shoot regeneration was MS+BA 1.5 mg/L+NAA 0.1 mg/L.When the shoots were cultured on a medium of 1/2MS+IBA 1.0 mg/L,more than 94.9% rooted and survived to develop thick shoots.The best potting media for subsequent ex-vitro development of the tissue culture plantlets comprised 2∶1 of peat and composted coconut powder.Conclusion] This study provided references for tissue culture seedlings of Polysics balfouriana.