| 北沙参外植体消毒方法研究 |
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| 引用本文： | 李颖,张钦德,李庆典.北沙参外植体消毒方法研究[J].安徽农业科学,2010,38(5):2336-2338. |
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| 作者姓名： | 李颖 张钦德 李庆典 |
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| 作者单位： | 青岛农业大学,山东青岛,266109;山东中医药高等专科学校,山东烟台,264000 |
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| 基金项目： | 山东省中医药科技发展计划重点项目(2007Z006-3) |
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| 摘 要： | 分别以北沙参田间栽培苗及温室盆栽苗的幼嫩叶片、田间高畦栽培苗的幼嫩根尖和温室水培苗的幼嫩叶柄为外植体,研究不同消毒组合对外植体污染率的影响。结果表明,75%酒精消毒20 s后再用0.1%升汞消毒5 min,接种外植体的存活率较高,且污染率较低。
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| 关 键 词： | 北沙参 外植体 消毒方法 污染率 |
Study on Disinfection Method of Glehnia littoralis Explants |
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| LI Ying et al.Study on Disinfection Method of Glehnia littoralis Explants[J].Journal of Anhui Agricultural Sciences,2010,38(5):2336-2338. |
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| Authors: | LI Ying |
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| Institution: | Qingdao Agricultural University;Qingdao;Shandong 266109 |
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| Abstract: | With tender leaves of cultivated Glehnia littoralis seedlings in field and greenhouse,tender root tip of cultivated seedlings in field with high ridge and tender petiole of water-cultured seedlings in greenhouse as the explants,the effect of different disinfection combinations on contamination rate of explants was studied.The results showed that the survival rate of explants treated by 75% alcohol for 20 s and then by 0.1% mercuric chloride for 5 min was the highest with the lowest contamination rate. |
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| Keywords: | Glehnia littoralis Explants Disinfection method Contamination rate |
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