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云南省景洪地区大茶树咖啡碱和儿茶素含量的比较
引用本文:段志芬,成浩,尚卫琼,李友勇,杨毅坚,杨盛美,刘本英,矣兵,郭顺云.云南省景洪地区大茶树咖啡碱和儿茶素含量的比较[J].安徽农业科学,2016(27).
作者姓名:段志芬  成浩  尚卫琼  李友勇  杨毅坚  杨盛美  刘本英  矣兵  郭顺云
作者单位:1. 云南省农业科学院茶叶研究所,云南勐海,666201;2. 中国农业科学院茶叶研究所,浙江杭州,310008;3. 云南省景洪市农业局经济作物工作站,云南景洪,666101
基金项目:国家自然科学基金项目(31160175,31440034);农业部茶树生物学与资源利用重点实验室开放课题。
摘    要:目的]对云南省景洪地区大茶树资源进行调查研究,以儿茶素类和咖啡碱含量为筛选指标,为筛选优异茶树种质资源提供依据。方法]采用高效液相色谱法对云南省景洪地区49株大茶树的咖啡碱和儿茶素含量进行测定和比较。结果]景洪地区大茶树儿茶素总含量的变化范围为9.500%~21.137%,酯型儿茶素含量的变化范围为0.085%~10.045%,其中表没食子儿茶素没食子酸酯(EGCG)含量的变化范围为3.717%~8.977%。非酯型儿茶素含量的变化范围为1.297%~4.346%;咖啡碱含量的变化范围为0.55%~4.76%;14株大茶树的EGCG含量均大于7.000%,其中10株为低咖啡碱茶树资源(咖啡碱含量≤1.50%)。结论]该研究结果对云南地区选育优异茶树种质资源具有一定的指导意义。

关 键 词:大茶树  儿茶素  咖啡碱  高效液相色谱

Comparative Analysis of Catechin and Caffeine Content of Tea Plant in Jinghong Region of Yunnan Province
Abstract:Objective] To investigate the tea plant Camellia sinensis (L.) O.Kuntze] resources in Jinghong Region of Yunnan Province,to use catechin and caffeine contents as the screening indexes,and to provide references for the screening of specific tea germplasm resources. Method] High-performance liquid chromatography was used to detect the contents of catechin and caffeine in tea samples.catechin and caffeine contents in 49 tea trees were compared in Jinghong Region of Yunnan Province.Result] The total catechin content ranged from 9.500% to 21.137%;the ester catechins content ranged from 0.085%to 10.054%.Among them,the EGCG content varied from 3.717%to 8.977%,the nonesterified cat-echin content ranged from 1.297% to 4.364%; the caffeine content ranged from 0.550% to 4.760%.EGCG in 14 tea trees were greater than 7.000%.Among them,10 were low caffeine tree resources (caffeine content≤1.50%).Conclusion] This research results has certain guiding significance to the breeding of excellent tea germplasm resources in Yunnan area.
Keywords:Tea plant (Camellia sinensis)  Catechin  Caffeine  HPLC
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