| 四棱豆杂交关键技术与杂交一代的分子鉴定研究 |
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| 引用本文： | 全妙华,蒋向辉,陈东明.四棱豆杂交关键技术与杂交一代的分子鉴定研究[J].安徽农业科学,2007,35(14):4140-4141,4143. |
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| 作者姓名： | 全妙华 蒋向辉 陈东明 |
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| 作者单位： | 怀化学院生物工程系,湖南怀化,418008;怀化学院生物工程系,湖南怀化,418008;怀化学院生物工程系,湖南怀化,418008 |
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| 基金项目： | 湖南省科研计划重点资助项目（05A064）;湖南省教育厅科研计划基金项目（05C693）. |
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| 摘 要： | 从花朵的选择、花粉粒的观察、去雄时间的对比、授粉技术的把握等方面对四棱豆杂交的关键技术进行了探索,找到了一条适合四棱豆杂交的技术路线,并利用RAPD技术进行了杂交一代的鉴定.研究结果表明:K0026×中翼1号杂交组合的杂交后代(F1)具备了母本和父本的各1条特异带.
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| 关 键 词： | 四棱豆 杂交技术 RAPD 杂种鉴定 |
| 文章编号： | 0517-6611（2007）14-04140-02 |
| 收稿时间： | 2007-02-16 |
| 修稿时间： | 2007-02-16 |
Key Hybridization Technology of Winged Bean and Molecular Identification of the F1 Hybrids |
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| QUAN Miao-hua et al.Key Hybridization Technology of Winged Bean and Molecular Identification of the F1 Hybrids[J].Journal of Anhui Agricultural Sciences,2007,35(14):4140-4141,4143. |
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| Authors: | QUAN Miao-hua |
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| Institution: | Department of Bioengineering, Huaihua University, Huihua, Hunan 418008 |
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| Abstract: | The key hybridization technology of winged bean was studied including flower selection,pollen observation and the best stage for emasculation.A suitable technology routes for hybridizing winged bean were found out.And the F1 hybrids were identified by RAPD.Result showed that the first offspring(F1) of K0026 and Zhongyi No.1 had the special bands of the male parent and the female parent. |
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| Keywords: | Winged bean Hybridization technology RAPD Hybrid identification |
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