HPLC指纹图谱在附子药材道地性鉴别中的应用研究

目的]研究附子药材的HPLC指纹图谱,并采用该方法作为区分附子道地和非道地药材的参考,建立附子药材的质量控制方法。方法]色谱条件为:色谱柱为Waters XB-C18色谱柱(250 mm×4.6 mm,5μm),柱温30℃,流动相为40 mmol/L醋酸铵(氨水调节pH10.0)-乙腈梯度洗脱,流速1.0 ml/min,检测波长为240 nm,分析时间为70 min,进样量20μl。结果]道地产区6批附子药材样品的化学成分比非道地产区要复杂,共确定出了22个共有峰,相似度较高,均在0.926以上,但从共有峰的峰面积比值来看,不同产地附子所含成分的含量有一定差异;非道地产区6批附子药材样品中存在15个共有峰,药材化学成分比道地产区要简单,相似度相对较差,均小于0.879,说明各非道地产区药材差异明显。结论]HPLC指纹图谱稳定性和重复性较好,能很好地区分道地和非道地附子药材,为更好地控制附子药材内在质量提供科学依据。

Study on the Application of HPLC Fingerprint in Genuineness Identification of Genuine Regional RADIX ACONITI LATERALIS PRAEPARATA

Objective] To study the high performance liquid chromatogram(HPLC) fingerprint chromatogram of RADIX ACONITI LATERALIS PRAEPARATA and apply it to identify the genuine regional and non-genuine regional medicinal herb.Method] The HPLC analysis was performed on a Waters XB-C18(250 mm×4.6 mm,5 μm) with the detection wavelength at 240 nm and column temperature at 30 ℃.Following the injection of 20 μl of sample solution,an elution with the mixture of 40 mmol/L ammonium acetate(adjusted to pH 10.0) and acetonitrile as mobile phase was initiated at a flow rate of 1 ml/min,about lasted 70 min.Result] A total of 22 common peaks were identified from the fingerprints of 6 bathes of genuine regional medicinal herbs,and their similarity was all above 0.926%.From the perspective of peak area ratio of common peaks,there were certain differences in the chemical constituents among different genuine regional medicinal herbs.The 6 batches of non-genuine regional medicinal herbs had 15 common peaks with the similarity all less than 0.879,which were significantly different in chemical constituents.In addition,the chemical constituents were more complicated in genuine regional than non-genuine regional medicinal herb.Conclusion] The established HPLC fingerprint could distinguish the genuine regional and non-genuine regional medicinal herb,which could provide scientific basis to better control the inner quality of RADIX ACONITI LATERALIS PRAEPARATA.