中国不同地区荔枝品种的RAPD分析

目的]对不同地区荔枝品种进行RAPD分析,拟通过该研究为荔枝品种体系分类及育种工作奠定基础。方法]以采自四川、广西、广东、福建和海南5个地区的荔枝品种为试验材料,通过改进的CTAB法提取其叶片总DNA,选取20对重复性好的随机引物进行RAPD扩增,并对该5个荔枝品种进行多态性和亲缘关系分析。结果]5个荔枝品种多态性为61%,其相似系数为0.571 4～0.803 6,其中广东和广西的相似系数最大,为0.803 6,最小的是四川和海南,为0.571 4。适当延长退火到延伸的时间(即ramp),将其调为0.3℃/s时,可显著提高RAPD的分辨率和产量。结论]该研究为荔枝品种体系分类以及进一步的育种研究提供了理论基础。

RAPD Analysis of Litchi chinensis Sonn.from Different Origins of China

Objective] To lay the foundation for the classification and breeding of Litchi(Litchi chinensis Sonn.) through RAPD(Random Amplification Polymorphic DNA) analysis of the Litchi cultivars with different origins.Method] 5 Litchi cultivars from Sichuan,Guangxi,Guangdong,Fujian and Hainan were used as experimental materials to extract genomic DNA by modified CTAB method.Twenty primers with well reproducibility were randomly selected to conduct RAPD amplification to analyze the polymorphism and genetic relationship of the five cultivars.Result] The polymorphism of the five Litchi cultivars was 61%,with the similarity coefficient of 0.571 4-0.803 6.The similarity coefficient of Guangdong and Guangxi was the largest of 0.803 6,and Sichuan and Hainan was the lowest of 0.571 4.The resolution and production of RAPD was significantly improved by appropriately prolonging the ramp time from annealing to extension to 0.3 ℃/s.Conclusion] This study provided theoretical basis for the classification and breeding of Litchi.