| 高聚物型高效液相色谱柱在依替米星发酵液分析中的应用 |
| |
| 引用本文： | 尹亚萍,魏荣卿,刘晓宁,王瑾.高聚物型高效液相色谱柱在依替米星发酵液分析中的应用[J].安徽农业科学,2010,38(26):14203-14205. |
| |
| 作者姓名： | 尹亚萍 魏荣卿 刘晓宁 王瑾 |
| |
| 作者单位： | 南京工业大学生物与制药工程学院色谱中心,江苏南京,210009;南京工业大学生物与制药工程学院色谱中心,江苏南京,210009;南京工业大学生物与制药工程学院色谱中心,江苏南京,210009;南京工业大学生物与制药工程学院色谱中心,江苏南京,210009 |
| |
| 摘 要： | 目的]建立一种用聚苯乙烯反相色谱柱联用示差检测器高效液相色谱法（HPLC）分析依替米星发酵液的方法。方法]使用MKF—RP—ETI色谱柱（100．0mm×7．8mm，200．0mm×7．8mm，5μm），流动相：O．22mol／L醋酸盐缓冲液（pH=6．0），流速：1ml／min，检测器：示差检测器。结果]MKF—RP—ETI色谱柱和HPLC—RD法可方便、快速用于分析鉴定依替米星发酵液，依替米星保留时间约为8min。结论]该方法可方便、快速、高效地用于依替米星发酵液生产、纯化过程中在线检测的分离分析。
|
| 关 键 词： | 聚苯乙烯反相色谱柱 高效液相色谱法 依替米星 |
Separation of Etimicin Fermentation Liquor by High Performance Liquid Chromatographic Column Packed with Polymer Resin |
| |
| Institution: | YIN Ya-ping et al ( Center of Chromatography, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, Jiangsu 210009) |
| |
| Abstract: | Objective] The aim was to establish a method for the isolation of Etimicin fermentation liquor by polystyrene reverse phase column(MKF-RP-ETI)with High Performance Liquid Chromatographic(HPLC).Method] The MKF-RP-ETI column was used to separate Etimicin fermentation liquor.The mobile phase was 0.22 mol/L acetate buffer(pH=6.0).The flow velocity was 1 ml/min.The detector was refractive index detector(RD).Result] The MKF-RP-ETI column with HPLC-RD showed satisfactory features in separation properties and the retention time of Etimicin was as long as 8 minutes.Conclusion] This method can be applied for rapid and efficient purification and isolation of Etimicin fermentation liquor. |
| |
| Keywords: | MKF-RP-ETI column HPLC Etimicin |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
