| 含乳饮料中山梨酸·苯甲酸·糖精钠的含量测定前处理方法初探 |
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| 引用本文： | 杨洁,夏虹,彭茂民.含乳饮料中山梨酸·苯甲酸·糖精钠的含量测定前处理方法初探[J].安徽农业科学,2007,35(25):7729-7730,7753. |
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| 作者姓名： | 杨洁 夏虹 彭茂民 |
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| 作者单位： | 湖北省农业科学院农业测试与科技信息中心,湖北武汉,430064;湖北省农业科学院农业测试与科技信息中心,湖北武汉,430064;湖北省农业科学院农业测试与科技信息中心,湖北武汉,430064 |
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| 摘 要： | 探讨含乳饮料中山梨酸、苯甲酸、糖精钠的HPLC含量测定方法,在比较5种沉淀样品中蛋白质的方法后,确定以亚铁氰化钾与硫酸锌溶液为沉淀剂,采用C18反相柱,以甲醇:乙酸铵溶液(0.02 mol/L)=15∶85为流动相,紫外230 nm检测,结合保留时间及二极管阵列检测器(PDA)光谱分析信息定性。添加水平为60 mg/kg时山梨酸、苯甲酸、糖精钠的平均回收率范围为92.85%~98.16%,变异系数小于2.7%。结果表明,该法简单、快速、灵敏,适用于含乳饮料中山梨酸、苯甲酸、糖精钠的含量测定。
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| 关 键 词： | 山梨酸 苯甲酸 糖精钠 含乳饮料 前处理方法 高效液相色谱法 |
| 文章编号： | 0517-6611（2007）25-07729-02 |
| 修稿时间： | 2007-01-18 |
Investigation of Pretreatment Methods in Determination of Sorbic Acid,Benzoic Acid and Sodium Saccharin in Dairy Drink by HPLC |
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| YANG Jie et al.Investigation of Pretreatment Methods in Determination of Sorbic Acid,Benzoic Acid and Sodium Saccharin in Dairy Drink by HPLC[J].Journal of Anhui Agricultural Sciences,2007,35(25):7729-7730,7753. |
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| Authors: | YANG Jie |
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| Institution: | Center of Agricultural Test and Scientific Information, Hubei Academy of Agricultural Sciences, Wuhan, Hubei 430064 |
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| Abstract: | A HPLC method for determination of sorbic acid(SA),benzoic acid(SB) and sodium saccharin(Sac) in dairy drink was studied.The comparison of five methods of precipitating protein showed that the solution of potassium ferrocyanide and zinc sulfate as protein's precipitant was appropriate.The sample was analyzed on a C18 reversed-phase column with a mobile phase of methanol and ammonium acetate(15∶85,V/V).The recovery of 60 mg/kg SA,SB and Sac was 92.85%～98.16%.The variation coefficient was less than 2.7%.This method was simple,rapid,sensitive.And it could be used in the determination of SA,SB and Sac in dairy drink. |
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| Keywords: | Sorbic acid Benzoic acid Sodium saccharin Dairy drink Pretreatment method HPLC |
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