| 野葛愈伤组织诱导及分化的研究 |
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| 引用本文： | 洪森荣,尹明华.野葛愈伤组织诱导及分化的研究[J].安徽农业科学,2007,35(12):3493-3494. |
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| 作者姓名： | 洪森荣 尹明华 |
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| 作者单位： | 上饶师范学院生命科学系,江西上饶,334001;上饶师范学院生命科学系,江西上饶,334001 |
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| 基金项目： | 江西上饶师范学院科技项目（2006-2007）. |
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| 摘 要： | 对野葛叶片和茎段愈伤组织的诱导及分化进行了研究,结果表明:6-BA 2 mg/L+NAA 0.5 mg/L对叶片愈伤组织的诱导效果最好,6-BA 2 mg/L+NAA 1 mg/L对茎段愈伤组织的诱导效果最好,出愈率均达100%;6-BA 2 mg/L+NAA 1 mg/L有利于叶片形成的愈伤组织分化为芽,6-BA 2mg/L+NAA 0.5 mg/L则有利于茎段形成的愈伤组织分化为芽.
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| 关 键 词： | 野葛 愈伤组织 再分化 芽 |
| 文章编号： | 0517-6611（2007）12-03493-02 |
| 收稿时间： | 2007-01-17 |
| 修稿时间： | 2007-01-17 |
Study on the Callus Induction and Differentiation of Pueraria lobata |
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| HONG Sen-Rong ,et al.Study on the Callus Induction and Differentiation of Pueraria lobata[J].Journal of Anhui Agricultural Sciences,2007,35(12):3493-3494. |
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| Authors: | HONG Sen-Rong |
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| Institution: | Life Science Department,Shangrao Nonnal College, Shangrao, Jiangxi 334001 |
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| Abstract: | The object is to explore the effect of the plant growth substance on the callus induction and differentiation of Pueraria lobata.The explants used in culture were stem and leaf of Pueraria lobata.The results showed to the leaf,the best medium induced callus was MS basal medium containing 2 mg/L BA and 0.5 mg/L NAA;to the stem,the best was MS basal medium containing 2 mg/L BA and 1 mg/L NAA;to the induction of buds from leaf formative callus,the best was MS basal medium containing 2 mg/L BA and 1 mg/L NAA;to the induction of bud from stem formative callus,the best was MS basal medium containing 2 mg/L BA and 0.5 mg/L NAA. |
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| Keywords: | Pueraria lobata Callus Differentiation Bud |
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