高效液相色谱法测定竹叶提取物中木樨草素的含量

目的]建立高效液相色谱测定竹叶提取物中木樨草素含量的方法。方法]采用高效液相色谱法测定竹叶提取物中木樨草素的含量,其色谱条件是：色谱柱为YMC-ODS-A（4.6 mm×250.0 mm,5μm）,流动相为甲醇∶0.5%磷酸缓冲液梯度洗脱,流速为1.0 m l/m in,柱温为25℃,进样量为10μl,检测波长为335 nm。对测得的木樨草素进行系统适应性和专属性、线性关系及精密度的考察以及稳定性和添加回收试验。结果]按照上述的色谱条件进样,得到木樨草素的保留时间在38 m in左右,样品在38 m in也有明显的峰,说明色谱系统的适应性和专属性良好。木樨草素在0.2~14.0μg/m l与峰面积具有良好的线性关系（R2=0.999 6）,平均回收率为112.74%,RSD为2.45%。结论]该方法能较简单方便地测定竹叶黄酮提取物中木樨草素的含量。

Determination of Luteolin from Extract of Bamboo Leaves by HPLC

Objective]The study aimed to establish the method for determining the luteolin content from the extract of bamboo leaves by HPLC.Method]HPLC was used to determine the luteolin content from the extract of bamboo leaves and its chromatographic conditions were as follows: the chromatographic column was YMC-ODS-A(4.6 mm×250.0 mm,5 μm),the flowing phase was methanol∶0.5% phosphatic acid,with the flow speed of 1.0 ml/min,column temperature of 25 ℃ by linear gradient elution,sample-inject volume of 10 ul and detection wavelength of 335nm.The detected luteolin content was made for the investigation of system suitability and specificity,linear relationship and the precision and also for the tests of stability and adding recovery.Result]The sample was separated according to the above conditions of chromatography,the luteolin obtained had the retention time of about 38 min,and the sample at 38 min also had the obvious peak,showing that the adaptability and specificity of chromatographic system were good.The luteolin had good linear relationship(R2 = 0.999 6) with the peak area in 0.2~14.0 μg/ml range,with the average recovery of 112.74% and RSD of 2.45%.Conclusion]The method used for the determination of luteolin was simple and convenient.