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| 辐射诱变高产虫草素蛹虫草菌株的研究 | |
| 引用本文： | 张红,于桂英,徐方旭,王升厚.辐射诱变高产虫草素蛹虫草菌株的研究[J].安徽农业科学,2011,39(27):16575-16576,16696. |
| 作者姓名： | 张红 于桂英 徐方旭 王升厚 |
| 作者单位： | 1. 河北联合大学生命科学学院,河北唐山,063009 2. 沈阳师范大学实验中心,辽宁沈阳,110034 3. 沈阳师范大学特种菌业研究所,辽宁沈阳,110034 |
| 基金项目： | 辽宁省自然科学基金项目(20072058) |
| 摘 要： | 目的]筛选高产虫草素蛹虫草菌株。方法]采用放射性元素60Co-γ射线辐射诱变方法对蛹虫草菌株进行处理。结果]筛选出yccGy1016诱变菌株为目标菌株,其生物转化率达12.5%,菌丝中虫草素含量达481.6 mg/kg,子实体虫草素含量达9 600 mg/kg,明显高于对照菌株。结论]经10代加富PDA斜面继代培养及罐头瓶小麦培养基栽培试验,yccGy1016诱变菌株具有产量性状稳定、产生虫草素能力强的特点。 |
| 关 键 词： | 蛹虫草 虫草素 辐射育种 |
Study of High-yielding Cordycepin Strains of Cordyceps militaris by Radioactive Irradiation |
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| ZHANG Hong et al.Study of High-yielding Cordycepin Strains of Cordyceps militaris by Radioactive Irradiation[J].Journal of Anhui Agricultural Sciences,2011,39(27):16575-16576,16696. | |
| Authors: | ZHANG Hong |
| Institution: | ZHANG Hong et al(College of Life Science,Hebei United University,Tangshan,Hebei 063009) |
| Abstract: | Objective] The aim of the study was to screen high-yielding strains of Cordyceps militaris.Method] A method of radioactive 60Co-γ ray irradiation on the strain of Cordyceps militaris were used.Result] yccGy1016 mutant strain were selected and the biological conversion rate was 12.5%,mycelia of Cordyceps militaris content reached 481.6 mg/kg,sub-Entity cordycepin militaris content was 9 600 mg/kg,significantly higher than the original control strains.Conclusion] After 10 generations PDA slant enrichment subculture medium and cans bottles wheat cultivation experiment,the mutant strain was better than the original with stable yield characters and strong ability of producing cordycepin. |
| Keywords: | Cordyceps militaris Cordycepin Radiation breeding |
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