| 转化人参皂苷Re的活性菌株的鉴定 |
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| 引用本文： | 吴秀丽,刘成,陈靖.转化人参皂苷Re的活性菌株的鉴定[J].安徽农业科学,2011,39(36):22321-22322. |
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| 作者姓名： | 吴秀丽 刘成 陈靖 |
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| 作者单位： | 1. 宁夏医科大学药学院,宁夏回药现代化工程技术研究中心,宁夏银川750004
2. 宁夏医科大学科技中心,宁夏银川,750004 |
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| 摘 要： | 目的]筛选长白山人参土壤中的活性微生物，转化单体人参皂苷产生稀有抗肿瘤成份。方法]从长白山人参根际土壤中分离各类菌株，对单体人参皂苷Re进行微生物转化；结合菌落形态、产孢结构、孢子形态特征以及菌株ITSrDNA核酸序列分析，对活性菌株进行鉴定。结果]从长白山人参根际土壤中分离各类真菌菌株68株，其中菌株SRS一10对三醇组人参皂苷Re具有较强的转化活性。结论]阳性菌株SRS-10被鉴定为链格孢Alternaria alternata，能将人参皂苷Re转化为人参皂苷Rgl。
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| 关 键 词： | 人参皂苷Re 人参皂苷Rg1 链格孢 鉴定 |
Identification of the Active Strain of Ginsenoside Re |
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| Institution: | WU Xiu-li et al (Ningxia Research Center of Modern Hui Medicine Engineering and Technology,School of Pharmacy,Ningxia Medical University,Yinchuan,Ningxia 750004) |
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| Abstract: | Objective] To screen out the active microorganism in ginseng soil of Changbai Mountain,and transform the rare anti-tumor components produced by ginsenoside.Method] Various strains were isolated from the root soil of Changbai Mountain ginseng,microbiology of Re was transformed,the active strain was identified based on morphological characteristics of colonies,conidia and conidiophores,as well as ITS-5.8S rDNA sequences.Result] Totally 68 fungal strains were isolated and strain SRS-10 showed positive activity on G-Re.Conclusion] The strain SRS-10,identified as Alternaria alternate,could transform G-Re into G-Rg_1. |
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| Keywords: | Ginsenoside Re Ginsenoside Rg_1 Alternaria alternate Identification |
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