激素对水仙组培苗生根的影响

目的]确定水仙组培苗小鳞茎生根诱导的适宜激素种类和水平。方法]以水仙栽培品种Fortissimo的组织培养小鳞茎为试验材料,以1/2MS为基本培养基,设计1.0、0.5、0.10、.05 mg/L 4个NAAI、BA浓度,研究激素对水仙组培苗生根诱导的影响。结果]添加0.1mg/L NAA或0.1 mg/L IBA培养基处理的小鳞茎根生长健壮,生根诱导率高,与其他处理存在显著差异(P<0.05);添加0.05 mg/L IBA处理的根生长比较细短;添加1.0 mg/L NAA或0.5 mg/L IBA处理的小鳞茎生长较快,但对根的诱导效果不明显。结论]在1/2MS培养基中,加入0.1 mg/L NAA或0.1 mg/L IBA均对水仙组培苗的生根诱导有显著促进作用。

Effects of Hormone on the Rooting of Tissue Culture Seedlings in Narcissus

Objective] The research aimed to confirm the suitable hormone kinds and level for rooting induction of bulblet in tissue culture seedlings of narcissus.Method] Using the tissue cultured bulblet of narcissus cultivar Fortissimo as tested materials,with 1/2MS as the basic medium,4 concn.of NAA and IBA including 1.0,0.5,0.1 and 0.05 mg/L were set up to study the effects of hormone on rooting induction of tissue culture seedlings in narcissus.Result] The root of bulblets treated by the medium with adding 0.1 mg/L NAA or 0.1 mg/L IBA grew strongly with high rooting induction rate,which had significant difference with that of other treatments(P<0.05).The root in the treatment of adding 0.05 mg/L IBA was shorter and slimmer.The bulblets in the treatments of adding 1.0 mg/L NAA or 0.5 mg/L IBA grew faster,but its induction effect on the root was not obvious.Conclusion] Adding 0.1 mg/L NAA or 0.1 mg/L IBA to 1/2 MS medium both had the significant promotion on the rooting induction of tissue culture seedlings in narcissus.