HPLC法测定贯叶金丝桃中金丝桃素的含量

目的]用HPLC法测定贯叶金丝桃（Hypericum perforalurnL.）提取液中金丝桃素含量,以期为贯叶金丝桃的开发利用提供理论依据。方法]HPLC条件为：岛津VP-ODS C18（4.6 mm×150 mm,0.5μm）色谱柱;流动相为甲醇-乙酸乙酯-磷酸盐缓冲溶液（V∶V=60∶20∶20,pH=2.0）;流速为1.0 ml/min;柱温25℃;检测波长590 nm;进样量10μl。结果]贯叶金丝桃提取液在金丝桃素含量为2.56～19.20μg/ml浓度范围内与峰面积呈良好的线性关系（r=0.999 9）,测得金丝桃素的含量为0.79 mg/g,提取率0.079%。结论]该方法精密度良好,重现性好,稳定性好,适合贯叶金丝桃中金丝桃素含量的测定。

Determination of Contents of Hypericin in Hypericum perforatum L. by HPLC

Objective]To determine the content of hypericin in Hypericum perforalurn L.by HPLC,so as to provide references for its development and utihzation.Method]The content of hypericin was determined by HPLC,and HPLC conditions were as follows: column of SHIMADZU VP-ODS C18 column,mobile phase of methanol-ethyl acetate-phosphate buffer(V∶V=60∶20∶20,pH=2.0),flow rate of 1.0 ml/min,column temperature of 25 ℃,detection wavelength of 590 nm and injecting amount of 10 μl.Result]In the range of 2.56-19.20 μg/ml,the linear relationship of hypericin was good(r=0.999 9).The content of hypericin in Hypericum perforalurn L.was 0.79 mg/g and the yield of it was 0.079%.Conclusion]The method established in this study with good precision,reproducibility and stability is favorable for the determination of hypericin in Hypericum perforalurn L..