中国西北内陆干旱区大叶白麻野生居群遗传多样性研究

目的]研究大叶白麻野生居群的遗传多样性。方法]采用RAPD技术对分布于新疆、甘肃和青海的5个大叶白麻野生居群的遗传多样性水平进行检测。结果]经筛选的20条引物共扩增出165条清晰、重复性好的DNA条带,其中多态性条带为110条,多态位点百分率(PPB)为66.67%。居群间Nei′s多样性指数为0.2205,Shannon’s信息指数为0.3047,遗传分化系数(Gst)为0.9082。大叶白麻具有较高的遗传多样性水平,遗传分化主要发生在居群间。聚类分析显示居群间的相似度与分布区的地理和气候特征相关。结论]在利用大叶白麻资源的同时,应根据各居群的品质和遗传特征,对这些居群尤其是青海居群加以保护。

Genetic Diversity in Wild Populations of Poacynum hendersonii from Inland Arid Regions of Northwest China

Objective] Study on the genetic diversity in wild populations of Poacynum hendersonii.Method] Random amplified polymorphic DNA(RAPD) technique was employed to analyze the genetic diversity in five wild populations of P.hendersonii sampled from Xinjiang,Gansu and Qinghai province.Result] Totally 165 clear and repeatable bands were generated in RAPD reaction by using 20 primers screened from 80 primers,of which 110 were polymorphic,accounting for 66.67%.At species level,Nei′s gene diversity index(H),Shannon's information index(I) and genetic differentiation coefficient(Gst) were 0.220 5,0.304 7 and 0.908 2,respectively.P.hendersonii germplasm resources share a high level of genetic diversity,and genetic differentiation mainly exists among the populations.Results from genetic distances and cluster analysis showed that relationships among P.hendersonii populations were to some extent related with their geographical and climatic characters.Conclusion] This study suggests that the conservation of P.hendersonii should focus on the protection of many populations,particularly the Qinghai population.