| 核果类基因组DNA提取和RAPD条件优选 |
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| 引用本文： | 沈向,郑学勤.核果类基因组DNA提取和RAPD条件优选[J].山东农业大学学报(自然科学版),1999,30(2):154-160. |
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| 作者姓名： | 沈向 郑学勤 |
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| 作者单位： | 山东农业大学园艺系，西北农业大学园艺系，中国热带农业科学院热带作物生物技术国家重点实验室 |
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| 摘 要： | 采用３种方法分离杏、桃等核果类树种的基因组ＤＮＡ，比较了其分离效果。并以此为模板进行ＲＡＰＤ反应，调整ＲＡＰＤ反应体系组成和热循环条件，以进行该树种的系统分类研究。
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| 关 键 词： | 核果类 基因组DNA RAPD |
OPTIMIZATOIN OF STONE FRUITS GENOMIC DNAISOLATION AND RAPD CONDITIONS |
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| Shen XiangZheng XueqinRen XiaolinLi Jiarui.OPTIMIZATOIN OF STONE FRUITS GENOMIC DNAISOLATION AND RAPD CONDITIONS[J].Journal of Shandong Agricultural University,1999,30(2):154-160. |
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| Authors: | Shen XiangZheng XueqinRen XiaolinLi Jiarui |
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| Institution: | Shen Xiang1Zheng Xueqin3Ren Xiaolin2Li Jiarui2 |
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| Abstract: | Three methods were adopted to isolate genomic DNAs of stone fruit trees,such as apricot and peach.The results show that one of them is more effective. We have got the suitable conditions of RAPD system with the DNAs as template,which will be helpful to classify the species and cultivars of stone fruit trees. |
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| Keywords: | stone fruit(Prunus spp ) genomic DNA RAPD |
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