烟草赤星病菌遗传多样性的ISSR和RAPD标记比较分析

采用ISSR和RAPD 2种分子标记方法对来自不同地区的28份烟草赤星病菌进行遗传多样性分析,筛选后选用10个ISSR引物和10个RAPD引物,ISSR扩增出多态性条带112条,多态性条带百分率为86.82%,菌株间相似性系数为0.53～0.97;RAPD引物扩增出多态性条带70条,多态性条带百分率为81.39%,菌株间相似性系数为0.57～0.94;用SPSS17.0软件对2种标记遗传距离进行相关性分析,发现2种分子标记结果呈显著正相关,表明2种分子标记方法都适合于烟草赤星病菌遗传多样性研究,ISSR是一种多态性优于RAPD的标记技术。根据2种标记的结果,利用NTSYS软件按UPGMA方法进行聚类分析,发现烟草赤星病菌遗传多样性与地理差异没有显著相关性。

Comparison and Analysis of Molecular Methods ISSR and RAPD Used for Assessment of Genetic Diversity in Alternaria alternate

Molecular markers ISSR and RAPD were used to analyze the genetic diversity of 28 A.alternata.Through the primers tested,10 ISSR primers and 10 RAPD primers were selected for their reproducibility and high polymorphism.112 polymorphic bands were amplified by ISSR.The ratio of polymorphic band was 86.82 %.The coefficient of similarity between strains was 0.53-0.97;while 70 polymorphic bands were amplified by RAPD and the ratio of polymorphic band was 81.39 %.The coefficient of similarity between strains was 0.53-0.94.The correlation of genetic distance of two markers was analyzed with SPSS17.0 software.The results proved that these two markers were significantly correlated.The results revealed that ISSR and RAPD markers were suitable for assessment of genetic diversity of A.alternata,the ISSR marker was superior to the RAPD marker,and that there was little correlation between geographic origin and genetic diversity of A.alternate using NTSYS program by UPGMA cluster analysis.