大豆总DNA直接导入法培育优势高蛋白玉米材料研究

利用外源总DNA转导的花粉管通道法和经我们发展的减压渗透法将大豆总DNA导入玉米自交系统48-2和S37，在743份后代材料中筛选出8份高蛋白含量变异材料，它们的蛋白质与受体相比提高20%以上。用种子蛋白SDS-PAGE、谷氨酸-草酰乙酸同工酶及RAPD等分析方法对变异材料及其受体进行了鉴定分析。同时也对此8份变异材料的自交后代进行了筛选和S37在遗传上存在明显的差异。在其自交后代中仍有部分株系保持了主蛋白含量特性。此外，48-2的变异材料的植株形态、花药颜色上有明显变化。上述结果初步证明高蛋白特性是可遗传的，这为选育高蛋白玉米新自交系提供了新材料，也为利用外源DNA直接导入技术创造玉米新种质开辟了一条行这有效的新途径。

A study on the screening high-protein variants by transmitting exogenous soybean DNAs into maize

Eight high-protein variants were selected from 743 m aterialsobtained by means of transmitting the soybean DNA into maize inbred lin es 48-2 and S37 with pollen tube pathway technique and decompressed per meation.Through analyzing their seed protein,screening their inbreed progeny in laborators by means of seed protein SDS-PAGE,GOT isozyme and RAPD,it was found that there was greatly genetic diversity between the high-protein variants a nd receptors.At same time,the variants in plant pattern and anther color were si gnificantly different from receptors.The results indicated that the character of high-protein was hereditary,It can not only provide new materials for breeding high-protein maize inbreds lines,but also open a new effective way to use exog enous DNA introduction technique to create new maize material.