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丽江山慈姑秋水仙碱HPLC测定
引用本文:袁理春,徐中志,赵琪,吕丽芬,杨丽云,陈翠,周敏,周志宏,胥勇,杨永红.丽江山慈姑秋水仙碱HPLC测定[J].西南农业学报,2007,20(1):120-122.
作者姓名:袁理春  徐中志  赵琪  吕丽芬  杨丽云  陈翠  周敏  周志宏  胥勇  杨永红
作者单位:1. 云南省农业科学院高山经济植物研究所,云南,丽江,674100
2. 云南植物药业,云南,昆明,650019
3. 云南农业大学植物保护学院,云南,昆明,650201
基金项目:云南省科技厅项目重要濒危药材(丽江山慈菇)引种驯化研究(2002ZY-17)
摘    要:采用HPLC法测定丽江山慈姑鳞茎不同部位及云南省西北部和四川省越西县野生居群秋水仙碱含量。结果表明:丽江山慈姑地上部分基本不含秋水仙碱,鳞茎秋水仙碱含量由外及内依次降低,其中外膜质层为0.134%和鳞茎外层为0.171%;22个野生丽江山慈姑居群中,丽江红岩(GS01)含量最高,达0.61%,永胜板桥乡(GS22)最低,为0.13%,平均秋水仙碱含量为0.40%。

关 键 词:丽江山慈姑  HPLC法  秋水仙碱含量
文章编号:1001-4829(2007)01-0120-03
修稿时间:2006-04-15

Determination of colchiane in Iphigenia indica Kunth et Benth.by HPLC
YUAN Li-chun,XU Zhong-zhi,ZHAO Qi,LU Li-feng,YANG Li-yun,CHEN Cui,ZHOU Min,ZHOU Zhi-hong,XU Yong,YANG Yong-hong.Determination of colchiane in Iphigenia indica Kunth et Benth.by HPLC[J].Southwest China Journal of Agricultural Sciences,2007,20(1):120-122.
Authors:YUAN Li-chun  XU Zhong-zhi  ZHAO Qi  LU Li-feng  YANG Li-yun  CHEN Cui  ZHOU Min  ZHOU Zhi-hong  XU Yong  YANG Yong-hong
Institution:1. The Research Institute of Alpine Economic Plant, Yunnan Academy of Agricultural Sciences, Yunnan Lijiang 674100, China; 2. Yunnan Phytopharmaceutical Co. ,Ltd, Yunnan Kunming 650109, China ;3 Plant Protection College of Yunnan Agricultural University, Yunnan Kunming 650201, China
Abstract:Simulataneous HPLC determine the colchicine in different part of bulb of Iphigenia indica Kunth et Benth.and the wild population of the northwestward of Yunnan province and Yuexi county in Sichuan.The result shows:The up-ground parts of Iphigenia indica Kunth et Benth.contain few colchicine.The colchicine gradually reduce from outside to inner in the bulb of Iphigenia indica Kunth et Benth.,the outer-epidermis contain 0.134 %,the outer of bulb contain 0.171 %.The content of colchicine in the twenty-two wild populations GS01 is 0.61 % and GS22 is 0.13 %.The average conents of these colchicine is 0.40 %.
Keywords:Iphigenia indica Kunth et Benth    HPLC  content of colchicine
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