| 梨种质资源遗传多样性研究中的RAPD技术引物筛选 |
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| 作者姓名： | 马艳芝 张玉星 |
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| 作者单位： | 马艳芝,Ma Yanzhi(河北农业大学园艺学院,河北保定,071001;唐山师范学院生命科学系,河北唐山,063000);张玉星,Zhang Yuxing(河北农业大学园艺学院,河北保定,071001)
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| 摘 要： | 研究目的]RAPD技术已广泛用于种质资源遗传多样性研究,中国梨属植物资源丰富,通过对RAPD多态性引物的筛选,为RAPD技术在梨属种质资源中的应用提供参考;方法]以梨属44个种和品种为试验材料,通过RAPD扩增技术,筛选RAPD多态性引物;结果]从50个随机引物中筛选出18个多态性引物,所选出的多态性引物每个引物扩增出的条带数在4～11之间,扩增出的DNA片段分子量大多在450～2000 bp之间,共扩增出118个条带,其中样品间相同的条带数有11条,呈现多态性的条带107条,所选引物的多态百分率达90.8%.结论]所筛选的18个引物为梨属植物RAPD扩增多态性引物,可广泛用于梨属植物的RAPD扩增技术.
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| 关 键 词： | 梨 种质资源 遗传多样性 引物 |
Screening of Primers in Genetic Diversity Analysis of Pyrus Germplasm by RAPD |
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| Authors: | MA Yan-Zhi |
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| Abstract: | 18 primers selected from 50 ones were identified to be high polymorphic and were applied on RAPD amplification of 44 Pyrus plants, for genetic diversity analysis of Pyrus germplasm. The amounts of bands were 4-11 and the molecular weight mainly was 450bp-2000bp. A total of 118 DNA bands were amplified, including 11 same bands and 107 polymorphic bands. |
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| Keywords: | RAPD |
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