文心兰丛生芽组培快繁研究初报

笔者主要采用正交设计安排试验,探索通过以丛生芽途径再生植株的方法进行快速繁殖。研究的主要问题集中在不同基本培养基类型、不同激素配比和用量的选择、不同添加物的选择几个方面对丛生芽增值的影响。以期筛选出最优技术参数组合,提高丛生芽增殖系数,建立文心兰最优再生体系。结果表明:影响丛生芽增殖的显著因子分别是基本培养基、细胞分裂素BA、生长素NAA。最适宜的培养基配方是MS BA3.0mg/L或4.0mg/L NAA0.4mg/L或0.6mg/L 椰乳汁150.0ml/L或番茄汁150.0ml/L Vc0.2mg/L 活性炭1.0g/L 糖30.0g/L 琼脂8.0g/L pH5.4。

Study on the Technique System of Propagation of Bud Clumps in Oncidium

The regeneration way of bud clumps was adopted to establish a rapid technique system of propagation in Oncidium in this experiment. The main contents focus on the following aspects, such as choice of basic mediums, combination and density of hormone and addictions. The Orthogonal Design is adopted to arrange experiment. Through above research, it was tried to find the main factors and improve the propagation coefficient and build up the superior regeneration system in Oncidium in the paper. The result showed that the main factors of multiplication of bud clumps are the basic medium, BA and NAA. The best multiplication medium is MS BA 3.0 or 4.0 mg/L NAA 0.4 or 0.6 mg /L coconut milk 150.0 ml/L or tomato juice 150.0 ml /L active carbon 1.0 g /L Vc 0.2 mg /L active carbon 1.0g /L sugar 30.0 g /L agar 8.0g /L.