| 克新系列马铃薯遗传多样性的RAPD分析 |
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| 作者姓名： | 田国奎 李凤云 刘昭军 李庆全 王立春 盛万民 牛志敏 |
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| 作者单位： | 黑龙江省农科院马铃薯研究所 克山161606(田国奎，李凤云，李庆全，王立春，盛万民，牛志敏)，黑龙江省农科院生物技术研究中心 哈尔滨150086(刘昭军) |
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| 基金项目： | 黑龙江省农业科学院科研计划项目 |
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| 摘 要： | 利用RAPD标记技术对19份克新系列马铃薯品种进行了遗传多样性分析,分别提取19份马铃薯品种的DNA,进行随机引物多态性扩增,从1000条随机引物中初步筛选出7条有多态性的引物进行详细研究,每条RAPD引物扩增出4～9条带,共获得52条带,其中多态性条带为43条;19份马铃薯品种的遗传距离介于0.17～0.72之间,平均值为0.39,平均遗传距离介于0.31～0.51之间;聚类分析结果在GS=0.53处可将克新系列马铃薯品种划分为三类,聚类结果与系谱分析基本相符,同时也说明克新系列马铃薯的遗传基础有所拓宽。研究表明:RAPD标记简便、快速、成本低,适用于分析马铃薯遗传多样性,指导马铃薯育种实践中的亲本组配。
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| 关 键 词： | 马铃薯 聚类分析 遗传多样性 |
Genetic Diversity Analysis of KeXin Series Potato Cultivars by RAPD Markers |
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| Authors: | Tian GuoKui Li FengYun Liu ZhaoJun Li QingQuan Wang LiChun Sheng WanMin Niu zhimin |
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| Institution: | (1Potato Research Institute, Heilongjiang Academy of Agricultural Science , KeShan 161606;
2Biology Research Center, Academy of Agricultural Science in Heilongjiang Province ,Harbin 150086) |
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| Abstract: | The RAPD was used to study the genetic diversity of 19 KinXin series potato cultivars. The result showed that 52 RAPD bands were amplified using 7 primer, among them 43 bands were polymorphic, and 4~9 bands were amplified in each lane. The RAPD analysis indicated that the genetic distance of 19 potato cultivars was between 0.17~0.72, average value was 0.39. When all of the polymorphic bands were analysed by cluster analysis, 19 potato cultivars were classified into 3 groups. The analysis showed that there existed close sibship among the majority of potato cultivars, and the genetic distance between the minority cultivars and the others is longer, which indicate that the genetic basis of KeXin series potato cultivars is wide. The RAPD is more suitable in the diversity study of potato cultivars, and suitable to analyse the sibship of potato cultivars in breeding. |
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| Keywords: | RAPD |
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