| 三物白散对Survivin-RNA基因沉默转染胃癌SGC-7901细胞凋亡、迁移的影响 |
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| 作者姓名： | 范婧莹 娄余 张娜 彭瑶 朱莹 |
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| 作者单位： | 湖南中医药大学中西医结合学院, 湖南 长沙 410208,湖南中医药大学研究生院, 湖南 长沙 410208,湖南中医药大学第二附属医院, 湖南 长沙 410005,湖南中医药大学第二附属医院, 湖南 长沙 410005,湖南中医药大学第一附属医院, 湖南 长沙 410007 |
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| 基金项目： | 国家自然科学基金资助项目（81503561）；湖南省教育厅科研基金项目（16C1212）。 |
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| 摘 要： | 目的 探讨三物白散对Survivin-RNA基因沉默转染胃癌SGC-7901细胞凋亡、迁移的影响。方法 将实验分成6组：空白对照组、LV-RNAi 组、Survivin-RNAi-LV组、空白对照+中药组、LV-RNAi+中药组、Survivin-RNAi-LV+中药组；采用流式细胞仪检测各组细胞的凋亡，划痕实验检测各组细胞的迁移能力。结果 细胞凋亡率：Survivin-RNAi-LV组明显高于空白对照组和LV-RNAi组（P<0.05）；Survivin-RNAi-LV+中药组与空白对照+中药组、LV-RNAi+中药组比较明显较高（P<0.05）；Survivin-RNAi-LV+中药组高于Survivin-RNAi-LV组，差异具有统计学意义（P<0.05）。胃癌细胞的24 h迁移率：Survivin-RNAi-LV组明显低于空白对照组和LV-RNAi组（P<0.05）；Survivin-RNAi-LV+中药组与空白对照+中药组、LV-RNAi+中药组比较，其结果明显低于后两组（P<0.05）；Survivin-RNAi -LV+中药组与Survivin-RNAi-LV组比较，细胞迁移率明显降低，差异有统计学意义（P<0.05）。结论 Survivin基因沉默转染，增强了三物白散诱导胃癌SGC-7901细胞凋亡和抑制其迁移的作用。
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| 关 键 词： | Survivin 三物白散 胃癌 细胞凋亡 迁移 |
| 收稿时间： | 2018/9/4 0:00:00 |
Effects of Sanwubai Powder on Apoptosis and Migration of Gastric Cancer SGC-7901 Cells Transfected with Survivin-RNA Gene Silencing |
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| Authors: | FAN Jingying LOU Yu ZHANG N PENG Yao and ZHU Ying |
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| Institution: | College of Integrated Chinese and Western Medicines, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China,Graduate School, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China,The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China,The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China and The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China |
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| Abstract: | Objective To investigate the effects of Sanwubai Powder on apoptosis and migration of gastric cancer SGC-7901 cells transfected with Survivin-RNA gene silencing. Methods The experiment was divided into 6 groups:a blank control group, a LV-RNAi group, a Survivin-RNAi-LV group, a blank control group + TCM group, a LV-RNAi + TCM group, a Survivin-RNAi -LV + TCM group. The cell apoptosis was observed by flow cytometry, and the cell migration was assessed by cell scratch experiment. Results The apoptotic rate of the Survivin-RNAi -LV group was significantly higher than that of blank control group and LV-RNAi group (P<0.05); The apoptosis rate of the Survivin-RNAi-LV + TCM group was significantly higher than that of the blank control group + TCM group and the LV-RNAi + TCM group (P<0.05); The Survivin-RNAi-LV+ TCM group was higher than the Survivin-RNAi-LV group, and the difference was statistically significant (P<0.05). The 24 hour cell migration rate in the Survivin-RNAi -LV group was significantly lower than that in the blank control group and the LV-RNAi group (P<0.05). The Survivin-RNAi -LV + TCM group was significantly lower than that of the blank control group + TCM group and the LV-RNAi + TCM group (P<0.05); Compared with the Survivin-RNAi-LV group, the cell migration rate of the Survivin-RNAi-LV + TCM group was significantly decreased, and the difference was statistically significant (P<0.05). Conclusion After Survivin gene silencing transfection, the sensitivity of SGC-7901 cells to Sanwubai Powder was significantly enhanced, which further promoted the apoptosis of SGC-7901 cells and inhibited their migration. |
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| Keywords: | Survivin Sanwubai Powder gastric cancer apoptosis migration |
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