奶牛性控胚胎体外生产的影响因素及控制措施

针对奶牛胚胎体外生产环节,对体外受精和培养等技术进行了研究,旨在建立奶牛性控胚胎体外生产技术体系。结果表明,卵母细胞外周卵丘细胞至少保持3层以上,在成熟基础液中添加10ng/ml的EGF,利于核质成熟;在精子获能液中添加咖啡因降低精子的存活时间;牛胚胎前3d用CR1aa BSA培养,再用SOFaa 5?S培养,获得高的囊胚发育率,并且添加50ng/mlIGF-I后胚胎的发育得到了很大的改善。利用性控精液进行体外受精,受精率有轻微下降,但对生产的胚胎发育质量无明显(P>0.05)影响,胚胎移植后受胎率为40%左右。

Influence Factors and Control Measures for the in vitro Production of Sexed Embryos of Dairy Cattle

The present study was undertaken to establish the in vitro production system of sexed embryos of dairy cattle by optimizing culture system of the bovine embryo production, such as fertilization and embryo culture in vitro. The results showed that oocytes maintained at least three layers of cumulus were suitable of the karyoplasms maturation in matured medium added 10 ng/ml EGF during the maturation in vitro; the survival time of the sperm was decreased by the addition of caffeine. Embryos cultured with CRlaa added of BSA, followed in SOFaa with 5?S could develop high rate of blastocyst, and the embryo developmental percentage could be improved by the addition of 50ng/ml IGF-I. There was no direct influence on the developmental quality of the embryos fertilized in vitro with sexed semen although the fertilization rate had a slight drop (P<0.05), and the pregnant rate was about 40% after the embryos transferred.