| 大白菜胞质雄性不育线粒体基因特异分子RAPD标记及克隆 |
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| 作者姓名： | 冯冬林 |
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| 作者单位： | [1]福建农业职业技术学院,福建福州350007 [2]福州市蔬菜科学研究所,福建福州350003 [3]福建农林大学园艺学院,福建福州350002 |
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| 摘 要： | 用随机引物对大白菜细胞质雄性不育系及其保持系线粒体DNA的RAPD分析,在不育系与保持系的线粒体基因组间存在明显的差异,用引物S259在不育系中扩增并克隆得到特异片断(mt)S259-600bp,序列分析表明该片断与油菜线粒体基因NADH脱氢酶第四亚基序列有部分相似性,片段与雄性不育的关系还需进一步研究.
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| 关 键 词： | 大白菜 细胞质雄性不育 线粒体DNA |
RAPD Analysis of Mitochondrial DNA of Cytoplasmic Male Sterile Line and Its Maintainer in Chinese Cabbage |
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| Authors: | Feng Donglin Chen wenhui Lin yizhang Gao shan |
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| Abstract: | The mitochondrial DNA of Chinese cabbage CMS line and its maintainer line were analysed by the method of random amplified polymorphic DNA using random primers. And obtained clear amplification profile. Meanwhile, some obvious differences in mitochondrial genome were detected between CMS and maintainer line.And obtained specific amplified fragment (mt)S259~600bp of CMS in mitochondria DNA, the results revealed that and there are 23bp homology between (mt)S259~600bp and rape mitochondrion gene for NADH dehydrogenase subunit 4. The relationship of this different bands in CMS to male sterility needs further study. |
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| Keywords: | RAPD |
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