晋南牛父系和母系起源研究 |
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作者姓名: | 张志清 陈宏 刘波 雷初朝 张润锋 蓝贤勇 |
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作者单位: | 西北农林科技大学动物科技学院,陕西省农业分子生物学重点实验室,西北农林科技大学动物科技学院陕西省农业分子生物学重点实验室,西北农林科技大学动物科技学院陕西省农业分子生物学重点实验室,西北农林科技大学动物科技学院陕西省农业分子生物学重点实验室,西北农林科技大学动物科技学院陕西省农业分子生物学重点实验室,西北农林科技大学动物科技学院陕西省农业分子生物学重点实验室 陕西杨凌712100,陕西杨凌712100 徐州师范大学生物技术研究所,江苏徐州210029,陕西杨凌712100,陕西杨凌712100,陕西杨凌7 |
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基金项目: | 国家自然科学基金资助项目(30471238). |
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摘 要: | 为了研究晋南牛的父系起源和母系起源,随机抽取30头晋南公牛,分别对Y染色体INRA124微卫星位点和线粒体DNAcytb基因进行分析。Y染色体INRA124微卫星分析表明:30个个体中,具有132bp的等位基因约占总样本76.7%,表明晋南牛具有76.7%的普通牛父系血统;具有130bp的等位基因,约占总样本23.3%,表明晋南牛具有32.2%的瘤牛父系血统。cytb基因的HinfI酶切分析表明,在这31个个体中,没有发现牦牛和班腾牛的母系起源。
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关 键 词: | 晋南牛 Y染色体 Cytb 父系起源 母系起源 |
Research on the Male and Female Origin of Jinnan Cattle |
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Authors: | Zhang Zhiqing Chen Hong Liu Bo Lei Chuzhao Zhang Runfeng Lan Xianyong |
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Institution: | 1.College of Animal Science and technology, Northwest Science and Technology University of Agriculture and Forestry, Shaanxi key laborary of agricultural molecular biology, yangling shaaxi, 712100; 2. Instute of Biote chnology, XuZhou Normal University, |
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Abstract: | In order to investigate the male and female origin of Jinnan cattle, 30 male Jinnan cattle were selected and analyzed in INRA124 locus of Y chromosome and Cytb gene. The microsatellete analysis in INRA124 locus of Y chromosome showed there were 76.7% 132 bp allele in this locus, which showed that Nanyang cattle had 76.7% male origin of Bos taurus; there were 23.3% 130 bp allele in this locus, which showed Jinnan cattle had 23.3% male origin of bos indicus. The Hinf I digesting analysis of Cytb gene showed that there were no Yak and Banteng origin in these 30 cattles. |
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Keywords: | Jinnan cattle Y chromosome Cytb Male origin Female origin |
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