| 安徽野生香菇遗传多样性及杂种优势的RAPD分析 |
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| 作者姓名： | 王子迎 王书通 |
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| 作者单位： | 安徽教育学院生物系 合肥230061
(王子迎)，安徽农业大学植保系 合肥230036(王书通) |
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| 基金项目： | 国家自然科学基金资助项（30471124）. |
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| 摘 要： | 采用随机扩增多态性DNA(RAPD)技术,利用17个引物对26个安徽野生香菇菌株和6个香菇栽培品种进行了遗传多样性分析,并构建了遗传相关聚类图。在128个RAPD标记中,多态性标记为104个,占81.3%。聚类分析显示,当以相异距离0.25为阈值,32个菌株被划为5个RAPD遗传组,多数菌株之间遗传相似性较低。表明供试菌株在DNA水平上存在比较显著的遗传变异,具有较丰富的遗传多样性。杂种优势的检测表明亲本间遗传距离较大的组合其杂种优势也较强。
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| 关 键 词： | 安徽 香菇 RAPD 遗传多样性 杂交育种 |
Analysis of Genetic Diversity and Heterosis of Lentinula edodes strains in Anhui province of China Using RAPD |
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| Authors: | Wang Ziying Wang Shutong |
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| Institution: | 1.Dept. of Biology, A nhui Institute of Education, Hefei 230061; 2.Dept. Of Plant Protection Anhui Agricultural University, Hefei 230036 |
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| Abstract: | Using technique of random amplified polymorphic DNA (RAPD) assay 26 wild strains from Anhui province and 6 cultivated strains of Lentinula edodes in China were analyzed for genetic diversity with 17 primers. Among 128 amplified fragments 104(81.3%) were polymorphic. In 0.25 dissimilarity distance cluster analysis revealed the existence of five distinct genetic groups in which group. The results show that genetic similarity of these strains was low and there were obvious genetic variations in DNA level. The results revealed that high productive hybrids were obtained by crossing parents with larger genetic distances. |
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| Keywords: | Anhui Lentinula edodes RAPD Genetic diversity Crossbreeding |
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