| 石斛种质资源遗传多样性的RAPD分析 |
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| 作者姓名： | 任 羽 杨光穗 尹俊梅 詹艳玲 黄 先 |
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| 作者单位： | 中国热带农业科学院热带作物品种资源研究所 海南儋州571737(任羽，杨光穗，尹俊梅)，华南热带农业大学 海南儋州571737(詹艳玲，黄先) |
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| 摘 要： | 利用RAPD技术对10种石斛种质资源的遗传多样性及亲缘关系进行了分析。从100条10bp的RAPD引物中筛选获得17条多态性引物,对石斛属的10个种的基因组DNA进行扩增。共获得200条多态性带,平均每个引物产生11.8个多态性条带。材料间遗传相似系数变化范围为0.356～0.676。根据RAPD标记的结果,采用UPGMA法进行聚类分析,将石斛属的10个种区分开来,划分为4类。结果表明:RAPD标记技术较好地从分子水平上揭示石斛种质资源的遗传背景、亲缘关系。
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| 关 键 词： | 石斛 遗传多样性 |
Analysis of Genetic Diversity in Dendrobium Germplasm by RAPD Makers |
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| Authors: | Ren Yu Yang Guangsui Yin Junmei Zhan Yanling Huang Xian |
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| Institution: | 1 Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agriculture Sciences, Danzhou 571737; 2South China University of Tropical Agriculture, Danzhou 571737 |
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| Abstract: | The genetic diversity and relationship of 10 Dendrobium germplasm resources were analyzed by RAPD. The genomic DNA of 10 Dendrobium germplasms were tested with RAPD using 17 arbitrary 10-mer primers which were screened from 100 primers. The 200 polymorphic bands of RAPD bands were obtained.The average of polymorphic bands of per primer was 11.8. The RAPD based genetic similarity ranged from 0.356 to 0.676. UPGMA cluster analysis based on RAPD molecular markers data divided 10 species into four groups. The results showed that the RAPD approach was more efficient on Study of genetic background and relationship of Dendrobium germplasms . |
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| Keywords: | RAPD |
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