中蜂与意蜂营养杂交后代随机扩增多态DNA研究

以中华蜜蜂(Apis cerdna cerana Fabricius)和意大利蜜蜂(Apis mellifera ligustica Spinola)为实验材料,进行中华蜜蜂与意大利蜜蜂营养杂交,然后运用随机扩增多态DNA(RAPD)技术检测亲本和营养杂交后代的DNA多态性.结果表明:经过营养杂交,亲本蜜蜂与营养杂交子代的遗传相似系数变小,中华蜜蜂和意大利蜜蜂的特有DNA条带发生了转移.说明通过营养杂交,基因可能发生了转移.

Studies on the genetic diversity detected by RAPD in the offspring of the nutritional crossbreed between Apis cerana cerana and Apis mellifera ligustica

The experiments were conducted with Apis cerana cerana and Apis mellifera ligustica.In this study,nutritional hybridizations were carried out between Apis cerana cerana and Apis mellifera ligustica.Random amplified polymorphic DNA(RAPD) analysis was used to detect genetic diversity of the workers,which were the offspring of parent colonies and hybridization colonies.The results showed that the genetic similarity coefficient between the parent and the nutritional crossbreeding offspring tended to diminish.And some special bands were transferred mutually between Apis cerana cerana and Apis mellifera ligustica.All of these indicate the occurrence of probable gene transfer in honeybees after nutritional hybridization.