| 猪圆环病毒2型北京株全基因组的克隆与序列分析 |
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| 引用本文： | 汤世坤,张雪,周双海,王霞泰,吴国娟.猪圆环病毒2型北京株全基因组的克隆与序列分析[J].北京农学院学报,2009,24(2):47-50. |
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| 作者姓名： | 汤世坤 张雪 周双海 王霞泰 吴国娟 |
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| 作者单位： | 北京农学院,动物科学技术系,北京,102206;北京农学院,动物科学技术系,北京,102206;北京农学院,动物科学技术系,北京,102206;北京农学院,动物科学技术系,北京,102206;北京农学院,动物科学技术系,北京,102206 |
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| 基金项目： | 国家自然科学基金，北京市自然科学基金，北京市优秀人才培养专项资助项目，北京市属市管高校人才强教计划，北京市教委科技计划项目 |
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| 摘 要： | 为探索近年来北京地区猪圆环病毒2型（PCV2）的流行病学和变异规律，用PCR技术从北京不同猪场的临床发病猪和死亡猪病变组织中分别扩增和克隆获得4株PCV2分离株全基因组序列，并进行测定和分析。结果显示，4株PCV2基因组全长均为1767nt，其核苷酸同源性高达99％，与其他北京分离株的同源性也达98％以上，与国内外其他地区PCV2的核苷酸同源性也在95％以上。由此表明，各个PCV2分离株在进化方面存在地域相关性。
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| 关 键 词： | 猪圆环病毒2型 全基因组 克隆 序列分析 |
Cloning and Sequence Analysis of the Complete Genome of Porcine Circovirus Type 2 Beijing Strain |
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| TANG Shi-kun,ZHANG Xue,ZHOU Shuang-hai,WANG Xia-tai,WU Guo-juan.Cloning and Sequence Analysis of the Complete Genome of Porcine Circovirus Type 2 Beijing Strain[J].Journal of Beijing Agricultural College,2009,24(2):47-50. |
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| Authors: | TANG Shi-kun ZHANG Xue ZHOU Shuang-hai WANG Xia-tai WU Guo-juan |
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| Institution: | Department of Animal Science and Veterinary Medicine;Beijing University of Agriculture;Beijing 102206;China |
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| Abstract: | In order to explore the epidemiology and variance of porcine circovirus type 2(PCV2) in Beijing recently,the genome sequences of four PCV2 isolates were amplified by PCR assay with the tissues of the clinical diseased and dead pigs from several pig farms in Beijing.Then,the complete genomes of the four PCV2 isolates were cloned,sequenced and analyzed.The results showed that these genomes were all 1 767 nt in size,and shared above 99% identity of nucleotide.There were more than 98% identity of nucleotide bet... |
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| Keywords: | porcine circovirus type 2 genome cloning sequence analysis |
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