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| 蛹虫草菌丝体液体发酵培养条件的优化研究 | |
| 引用本文： | 程红艳,郭伟,常明昌,孟俊龙,杨杰.蛹虫草菌丝体液体发酵培养条件的优化研究[J].山西农业大学学报(自然科学版),2011,31(1):66-72. |
| 作者姓名： | 程红艳 郭伟 常明昌 孟俊龙 杨杰 |
| 作者单位： | 1. 山西农业大学资源环境学院,山西太谷030801;山西省食用菌工程技术研究中心,山西太谷030801 2. 山西省食用菌工程技术研究中心,山西太谷,030801 3. 山西省食用菌工程技术研究中心,山西太谷030801;山西农业大学食品科学与工程学院,山西太谷030801 |
| 基金项目： | 山西省科技攻关项目，山西省高校科技开发项目 |
| 摘 要： | 以蛹虫草菌丝体发酵液中的菌丝体干重和粗多糖产量为指标,筛选出液体培养基的最适碳源为蔗糖,最适氮源为黄豆粉。通过四因素三水平的正交试验,优化出液体发酵培养基的最佳配方为马铃薯20%,蔗糖5%,黄豆粉2%,磷酸二氢钾0.1%,硫酸镁0.1%,维生素B10.01%。并确定了摇瓶最优发酵工艺条件为装液量100 mL·250mL-1三角瓶,菌龄为72 h,接种量为15%,起始pH 6.0,温度24℃,摇床转速150 r·min-1,最适发酵周期为120 h。 |
| 关 键 词： | 蛹虫草 液体发酵 条件优化 |
Study of Optimization on Liquid Fermentation Conditions of Cordyceps militaris Mycelium |
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| CHENG Hong-yan,GUO Wei,CHANG Ming-chang,MENG Jun-long,YANG Jie.Study of Optimization on Liquid Fermentation Conditions of Cordyceps militaris Mycelium[J].Journal of Shanxi Agricultural University,2011,31(1):66-72. | |
| Authors: | CHENG Hong-yan GUO Wei CHANG Ming-chang MENG Jun-long YANG Jie |
| Institution: | CHENG Hong-yan1,2,GUO Wei2,CHANG Ming-chang2,3,MENG Jun-long2,YANG Jie2(1.College of Resource and Environment,Shanxi Agricultural University,Taigu Shanxi 030801,China,2.Shanxi Provincal Research for Engineering Technology of Edible Fungi,3.College of Food science and Engineering,China) |
| Abstract: | Compared with the dry weight of mycelium and the crude polysaccharide production,the optimal carbon source was sucrose,and the optimal nitrogen source was soybean powder.Through orthogonal experiment with four factors and three levels,the optimal fermentation medium of liquid was potato 20%,sucrose 5%,Soybean meal 2%,KH2PO4 0.1%,MgSO4·7H2O 0.1%,VB1 0.01%.And the best fermentation process control of flask was： filling liquid 100·250 mL-1,age of pure culture72 h,pitching 15%,initial pH 6.0,temperature 24 ℃,stirring rate 150 r·min-1,fermentation 120 h. |
| Keywords: | Cordyceps militaris Liquid fermentation Condition optimization |
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